在缺氧环境中产生的电外科烟雾的细胞毒性。
Cytotoxicity of electro-surgical smoke produced in an anoxic environment.
作者信息
Hensman C, Newman E L, Shimi S M, Cuschieri A
机构信息
Department of Surgery, University of Dundee, Ninewells Hospital and Medical School, Scotland, United Kingdom.
出版信息
Am J Surg. 1998 Mar;175(3):240-1. doi: 10.1016/s0002-9610(97)00291-2.
BACKGROUND
The effect on cell viability of smoke produced during high-frequency electro-surgery has not been previously reported. The aim of this study was to produce smoke in vitro, in a closed environment similar to that encountered in minimal access surgery, and to test its cytotoxic effects on cultured cells.
METHODS
Pig liver was cut repeatedly with an electro-surgical hook knife, and the smoke generated was collected and equilibrated with cell culture medium. MCF-7 human breast carcinoma cells were exposed briefly to various dilutions of this medium and tested for clonogenicity.
RESULTS
Electro-surgical smoke produced in a helium environment reduced the clonogenicity of the MCF-7 human breast carcinoma cells in a dose-dependent manner, falling to 30% when the cells were exposed to undiluted medium for 15 minutes.
CONCLUSIONS
We conclude that electro-surgical smoke is cytotoxic. The sublethal effects at lower dilutions are currently being investigated.
背景
高频电外科手术产生的烟雾对细胞活力的影响此前尚未见报道。本研究的目的是在类似于微创手术中遇到的封闭环境下体外产生烟雾，并测试其对培养细胞的细胞毒性作用。
方法
用电外科钩刀反复切割猪肝，收集产生的烟雾并用细胞培养基平衡。将MCF-7人乳腺癌细胞短暂暴露于该培养基的各种稀释液中，并测试其克隆形成能力。
结果
在氦气环境中产生的电外科烟雾以剂量依赖的方式降低了MCF-7人乳腺癌细胞的克隆形成能力，当细胞暴露于未稀释的培养基中15分钟时，克隆形成能力降至30%。
结论
我们得出结论，电外科烟雾具有细胞毒性。目前正在研究较低稀释度下的亚致死效应。
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