Tavormina P A, Burke D J
Department of Biology, University of Virginia, Charlottesville 22903, USA.
Genetics. 1998 Apr;148(4):1701-13. doi: 10.1093/genetics/148.4.1701.
The spindle checkpoint ensures accurate chromosome segregation by inhibiting anaphase onset in response to altered microtubule function and impaired kinetochore function. In this study, we report that the ability of the anti-microtubule drug nocodazole to inhibit cell cycle progression in Saccharomyces cerevisiae depends on the function of the kinetochore protein encoded by NDC10. We examined the role of the spindle checkpoint in the arrest in cdc20 mutants that arrest prior to anaphase with an aberrant spindle. The arrest in cdc20 defective cells is dependent on the BUB2 checkpoint and independent of the BUB1, BUB3, and MAD spindle checkpoint genes. We show that the lesion recognized by Bub2p is not excess microtubules, and the cdc20 arrest is independent of kinetochore function. We show that Cdc20p is not required for cyclin proteolysis at two points in the cell cycle, suggesting that CDC20 is distinct from genes encoding integral proteins of the anaphase promoting complex.
纺锤体检查点通过响应微管功能改变和动粒功能受损来抑制后期开始，从而确保染色体准确分离。在本研究中，我们报告抗微管药物诺考达唑抑制酿酒酵母细胞周期进程的能力取决于由NDC10编码的动粒蛋白的功能。我们研究了纺锤体检查点在cdc20突变体停滞中的作用，这些突变体在后期之前因异常纺锤体而停滞。cdc20缺陷细胞中的停滞依赖于BUB2检查点，且独立于BUB1、BUB3和MAD纺锤体检查点基因。我们表明，Bub2p识别的损伤不是过多的微管，并且cdc20停滞独立于动粒功能。我们表明，在细胞周期的两个时间点，细胞周期蛋白的蛋白水解不需要Cdc20p，这表明CDC20不同于编码后期促进复合体整合蛋白的基因。
