Guillamón J M, Sabaté J, Barrio E, Cano J, Querol A
Unitat d'Enologia del Centre de Referència de Tecnologia d'Aliments, Departament de Bioquímica i Biotecnologia, Escola d'Enologia de Tarragona. Universitat Rovira i Virgili, Tarragona, Spain.
Arch Microbiol. 1998 May;169(5):387-92. doi: 10.1007/s002030050587.
In this study, we identified a total of 33 wine yeast species and strains using the restriction patterns generated from the region spanning the internal transcribed spacers (ITS 1 and 2) and the 5.8S rRNA gene. Polymerase chain reaction (PCR) products of this rDNA region showed a high length variation for the different species. The size of the PCR products and the restriction analyses with three restriction endonucleases (HinfI, CfoI, and HaeIII) yielded a specific restriction pattern for each species with the exception of the corresponding anamorph and teleomorph states, which presented identical patterns. This method was applied to analyze the diversity of wine yeast species during spontaneous wine fermentation.
在本研究中，我们利用跨越内转录间隔区（ITS 1和2）及5.8S rRNA基因区域产生的限制性酶切图谱，共鉴定出33种葡萄酒酵母菌种和菌株。该rDNA区域的聚合酶链反应（PCR）产物在不同菌种间显示出高度的长度变异。PCR产物的大小以及用三种限制性内切酶（HinfI、CfoI和HaeIII）进行的限制性分析，为每个菌种产生了特定的限制性酶切图谱，但相应的无性型和有性型状态除外，它们呈现出相同的图谱。该方法被应用于分析自发葡萄酒发酵过程中葡萄酒酵母菌种的多样性。
