Corbu Viorica Maria, Csutak Ortansa
Department of Genetics, Faculty of Biology, University of Bucharest, 060101 Bucharest, Romania.
Microorganisms. 2022 Dec 22;11(1):37. doi: 10.3390/microorganisms11010037.
(1) Background: Wine yeast research offers the possibility of isolating new strains with distinct metabolic properties due to the geographical location of the vineyard and the processes used in winemaking. Our study deals with the isolation and identification of six yeasts from spontaneously fermented wine wort from Romania and their characterization as new potential starter culture for traditional beverages, for food industry or biomedicine. (2) Materials and methods: The isolates were identified using conventional taxonomy tests, phenotypic phylogeny analysis (Biolog YT), MALDI-TOF mass spectrometry, PCR-RFLP, and sequencing of the ITS1-5,8S-ITS2 rDNA region. The capacity of the yeasts to grow under thermal, ionic, and osmotic stress was determined. The safe status was confirmed by testing virulence and pathogenicity factors. Assays were performed in order to evaluate the growth inhibition of strains and determine the antimicrobial mechanism of action. (3) Results and discussions: The yeast isolates were identified as belonging to the , , , , and genera. All the isolates were able to develop under the tested stress conditions and were confirmed as safe. With the exception of CMGB-MS1-1, all the isolates showed good antimicrobial activity based on competition for iron ions or production of killer toxins. (4) Conclusions: The results revealed the resistance of our yeasts to environmental conditions related to industrial and biomedical applications and their high potential as starter cultures and biocontrol agents, respectively.
(1) 背景：由于葡萄园的地理位置和酿酒过程，葡萄酒酵母研究提供了分离具有独特代谢特性新菌株的可能性。我们的研究涉及从罗马尼亚自发发酵的葡萄汁中分离和鉴定六种酵母，并将其表征为传统饮料、食品工业或生物医学的新型潜在发酵剂。(2) 材料与方法：使用传统分类学测试、表型系统发育分析（Biolog YT）、基质辅助激光解吸电离飞行时间质谱、聚合酶链反应-限制性片段长度多态性以及ITS1-5,8S-ITS2核糖体DNA区域测序对分离株进行鉴定。测定了酵母在热、离子和渗透胁迫下的生长能力。通过测试毒力和致病因素来确认安全状态。进行了试验以评估菌株的生长抑制情况并确定抗菌作用机制。(3) 结果与讨论：酵母分离株被鉴定为属于、、、、和属。所有分离株都能在测试的胁迫条件下生长，并被确认为安全。除CMGB-MS1-1外，所有分离株基于对铁离子的竞争或产生杀伤毒素均表现出良好的抗菌活性。(4) 结论：结果揭示了我们的酵母对与工业和生物医学应用相关环境条件的抗性，以及它们分别作为发酵剂和生物防治剂的巨大潜力。
