Hens J, Nuydens R, Geerts H, Senden N H, Van de Ven W J, Roebroek A J, van de Velde H J, Ramaekers F C, Broers J L
Department of Molecular Cell Biology and Genetics, Universiteit Maastricht, P.O. Box 616, 6200 MD Maastricht, The Netherlands.
Cell Tissue Res. 1998 May;292(2):229-37. doi: 10.1007/s004410051054.
Neuroendocrine-specific protein (NSP) reticulons are expressed in neural and neuroendocrine tissues and cell cultures derived therefrom, while most other cell types lack NSP-reticulons. Three major subtypes have been identified so far, designated NSP-A, NSP-B, and NSP-C. We have investigated the correlation between the degree of neuronal differentiation, determined by morphological and biochemical criteria, and NSP-reticulon subtype expression. For this purpose, several human neuroblastoma cell lines, exhibiting different degrees of neuronal differentiation, were examined immuno(cyto)chemically. It became obvious that the expression of NSP-C, as detected by immunofluorescence microscopy and Western blotting, is most prominent in cell lines with a high degree of neuronal differentiation, such as LA-N-5. Such highly differentiated cells also express other neural and neuroendocrine markers, such as neural cell adhesion molecule (NCAM), neurofilament proteins, synaptophysin, and chromogranin. NSP-A was observed in all cell lines to a different extent. However, no clear correlation was observed with the degree of neuronal differentiation as defined by other neuronal and neuroendocrine markers or morphology. NSP-B could not be detected. The induction of neuronal differentiation with nerve growth factor, dbcAMP, and retinoic acid in the rat pheochromocytoma cell line PC12 and the human teratocarcinoma cell line hNT2, respectively, induced the expression of NSP-A and NSP-C in these cell lines parallel to the induction of neurofilament protein expression. It is concluded that NSP-C expression, in particular, is strongly correlated with neuronal differentiation.
神经内分泌特异性蛋白（NSP）网质蛋白在神经组织、神经内分泌组织以及由此衍生的细胞培养物中表达，而大多数其他细胞类型则缺乏NSP网质蛋白。到目前为止，已鉴定出三种主要亚型，分别命名为NSP - A、NSP - B和NSP - C。我们研究了通过形态学和生化标准确定的神经元分化程度与NSP网质蛋白亚型表达之间的相关性。为此，对几种表现出不同程度神经元分化的人神经母细胞瘤细胞系进行了免疫（细胞）化学检测。通过免疫荧光显微镜和蛋白质免疫印迹法检测发现，很明显NSP - C在高度神经元分化的细胞系（如LA - N - 5）中表达最为突出。这种高度分化的细胞还表达其他神经和神经内分泌标志物，如神经细胞黏附分子（NCAM）、神经丝蛋白、突触素和嗜铬粒蛋白。在所有细胞系中均不同程度地观察到了NSP - A。然而，未观察到其与由其他神经元和神经内分泌标志物或形态学定义的神经元分化程度之间存在明显相关性。未检测到NSP - B。分别用神经生长因子、双丁酰环磷腺苷（dbcAMP）和视黄酸诱导大鼠嗜铬细胞瘤细胞系PC12和人畸胎瘤细胞系hNT2的神经元分化，在这些细胞系中诱导NSP - A和NSP - C表达的同时，也平行诱导了神经丝蛋白的表达。得出的结论是，特别是NSP - C的表达与神经元分化密切相关。
