Yuri K, Nakata K, Katae H, Yamamoto S, Hasegawa A
Research Laboratories, Animal Science Division, Dainippon Pharmaceutical Co. Ltd., Osaka, Japan.
J Vet Med Sci. 1998 Mar;60(3):287-90. doi: 10.1292/jvms.60.287.
A variety of virulence factors (VFs) such as type 1 fimbriae, pilus associated with pyelonephritis, S fimbriae, afimbrial adhesin, alpha-hemolysin, aerobactin and cytotoxic necrotizing factor 1 are associated with uropathogenic Escherichia coli. In this study, 80 uropathogenic E. coli strains in 50 dogs and 30 cats suffering from UTI. In addition, 60 E. coli strains were isolated from fecal samples from 30 each of healthy dogs and cats. The distribution of VFs of uropathogenic E. coli strains isolated from dogs and cats suffering from urinary tract infections (UTI) were examined by the colony hybridization test with seven DNA probes specific for VFs, and the results were compared with those obtained in the studies on strains from humans with UTI. In uropathogenic E. coli strains isolated from dogs and cats suffering from UTI, VFs were detected as frequently as in the strains isolated from humans with UTI. Although less frequently, genes encoding these VFs especially pap, sfa, hly, and cnf 1 genes were also associated with E. coli strains isolated from feces of healthy cats, in contrast to the distribution pattern of uropathogenic E. coli observed in humans. Furthermore, all VFs except pil were significantly more frequently detected in strains isolated from urine of animals with cystitis than in those isolated from feces of healthy humans. These results indicate that VFs of E. coli contribute to the pathogenesis of UTI in dogs and cats.
多种毒力因子（VFs），如1型菌毛、与肾盂肾炎相关的菌毛、S菌毛、无纤毛黏附素、α-溶血素、气杆菌素和细胞毒素坏死因子1，都与尿路致病性大肠杆菌有关。在本研究中，从50只患有尿路感染的犬和30只患有尿路感染的猫中分离出80株尿路致病性大肠杆菌菌株。此外，从30只健康犬和30只健康猫的粪便样本中分别分离出60株大肠杆菌菌株。通过使用七种针对毒力因子的DNA探针进行菌落杂交试验，检测从患有尿路感染（UTI）的犬和猫中分离出的尿路致病性大肠杆菌菌株的毒力因子分布，并将结果与从患有UTI的人类菌株研究中获得的结果进行比较。在从患有UTI的犬和猫中分离出的尿路致病性大肠杆菌菌株中，毒力因子的检测频率与从患有UTI的人类中分离出的菌株相同。尽管频率较低，但编码这些毒力因子的基因，特别是pap、sfa、hly和cnf 1基因，也与从健康猫粪便中分离出的大肠杆菌菌株有关，这与在人类中观察到的尿路致病性大肠杆菌的分布模式不同。此外，除菌毛外，所有毒力因子在从患有膀胱炎的动物尿液中分离出的菌株中被检测到的频率明显高于从健康人类粪便中分离出的菌株。这些结果表明，大肠杆菌的毒力因子有助于犬和猫UTI的发病机制。
