Chiew Y F, Hall L M
Department of Medical Microbiology, St Bartholomew's, London, UK.
J Hosp Infect. 1998 Mar;38(3):223-30. doi: 10.1016/s0195-6701(98)90278-x.
Enterococci are frequently isolated as nosocomial pathogens and have often acquired intrinsic drug resistances. Molecular typing techniques have been developed to assist in epidemological and infection control measures. This study investigates enterococci with high-level aminoglycoside resistance (HLAR) from the National University Hospital (NUH) of Singapore, and evaluates and compares three methods for typing: restriction enzyme analysis by conventional gel electrophoresis [restriction fragment length polymorphism (RFLP)], pulsed-field gel electrophoresis (PFGE), and polymerase chain reaction (PCR) using random amplified polymorphic DNA (RAPD). Fifty-two isolates of Enterococcus faecalis and 13 isolates of Enterococcus faecium were used for the study. The numbers of patterns obtained for E. faecalis and E. faecium were 26 and 4, respectively by the RFLP method, and very similar discrimination was obtained by PFGE. RAPD PCR results were not reliably reproducible. A single pattern type by RFLP accounted for 16 of the E. faecalis isolates, suggesting hospital spread.
肠球菌常作为医院病原体被分离出来，且常常获得内在耐药性。分子分型技术已被开发用于协助流行病学和感染控制措施。本研究调查了来自新加坡国立大学医院（NUH）的具有高水平氨基糖苷类耐药性（HLAR）的肠球菌，并评估和比较了三种分型方法：通过传统凝胶电泳进行的限制性酶切分析[限制性片段长度多态性（RFLP）]、脉冲场凝胶电泳（PFGE）以及使用随机扩增多态性DNA（RAPD）的聚合酶链反应（PCR）。52株粪肠球菌分离株和13株屎肠球菌分离株用于该研究。通过RFLP方法，粪肠球菌和屎肠球菌获得的型别数量分别为26种和4种，PFGE获得了非常相似的鉴别结果。RAPD PCR结果不可靠且无法重复。RFLP的单一型别占粪肠球菌分离株的16株，提示在医院内传播。
