Christensen H, Olsen J E
Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
FEMS Microbiol Lett. 1998 Apr 1;161(1):89-96. doi: 10.1111/j.1574-6968.1998.tb12933.x.
DNA sequences covering 57% of atpD encoding the beta subunit of ATP synthase were determined for 16 strains of Salmonella enterica, two strains of S. bongori, and one strain each of Citrobacter freundii and Yersinia enterocolitica, and comparison was made with the published Escherichia coli and Enterobacter aerogenes sequences. The phylogenetic tree based on maximum-likelihood analysis showed separation of the subspecies of S. enterica except for two serotypes of subspecies II which were unsupported by a common node. The two serotypes of S. bongori were separated from S. enterica and related to the serotypes of subspecies II. A tight relationship was found between S. enterica subspecies IIIa consisting of monophasic serotypes and subspecies IIIb consisting of diphasic serotypes. This is in conflict with results obtained for most other housekeeping genes and the 23S rRNA gene separating mono- from diphasic subspecies.
测定了16株肠炎沙门氏菌、2株邦戈尔沙门氏菌、1株弗氏柠檬酸杆菌和1株小肠结肠炎耶尔森氏菌中编码ATP合酶β亚基的atpD基因57%的DNA序列，并与已发表的大肠杆菌和产气肠杆菌序列进行了比较。基于最大似然分析的系统发育树显示，除了亚种II的两个血清型没有共同节点支持外，肠炎沙门氏菌的亚种之间相互分离。邦戈尔沙门氏菌的两个血清型与肠炎沙门氏菌分离，并与亚种II的血清型相关。发现由单相血清型组成的肠炎沙门氏菌亚种IIIa和由双相血清型组成的亚种IIIb之间关系紧密。这与大多数其他管家基因和23S rRNA基因将单相亚种与双相亚种区分开来的结果相矛盾。
