Ludwig W, Neumaier J, Klugbauer N, Brockmann E, Roller C, Jilg S, Reetz K, Schachtner I, Ludvigsen A, Bachleitner M
Lehrstuhl für Mikrobiologie, Technische Universität München, Germany.
Antonie Van Leeuwenhoek. 1993;64(3-4):285-305. doi: 10.1007/BF00873088.
Comparative sequence analyses were performed on 14 genes encoding bacterial elongation factors EF-Tu and 7 genes encoding the beta-subunit of bacterial F1F0 type ATP-synthases. The corresponding predicted amino acid sequences were compared with published primary structures of homologous molecules. Phylogenetic trees were reconstructed from both data sets of aligned protein sequences and from an equivalent selection of 16S rRNA sequences by applying distance matrix and maximum parsimony methods. The EF-Tu data were in very good agreement with the rRNA data, although the resolution within the EF-Tu tree was reduced at certain phylogenetic levels. The resolution power of the ATPase beta-subunit sequence data were more reduced than those of the EF-Tu data. In comparison with the 16S rRNA tree there are minor differences in the order of adjacent branchings within the ATPase beta-subunit tree.
对编码细菌延伸因子EF-Tu的14个基因和编码细菌F1F0型ATP合酶β亚基的7个基因进行了比较序列分析。将相应的预测氨基酸序列与已发表的同源分子一级结构进行比较。通过应用距离矩阵和最大简约法，从比对后的蛋白质序列数据集以及16S rRNA序列的等效选择中重建系统发育树。尽管在某些系统发育水平上EF-Tu树的分辨率有所降低，但EF-Tu数据与rRNA数据非常吻合。ATPaseβ亚基序列数据的分辨率能力比EF-Tu数据降低得更多。与16S rRNA树相比，ATPaseβ亚基树中相邻分支的顺序存在微小差异。
