Delbac F, Duffieux F, David D, Metenier G, Vivares C P
LBCP, UPESA CNRS 6023, Université Blaise Pascal, Aubière, France.
J Eukaryot Microbiol. 1998 Mar-Apr;45(2):224-31. doi: 10.1111/j.1550-7408.1998.tb04529.x.
Microsporidia can form small spores with a unique invasive apparatus featuring a long polar tube whose extrusion allows entry of infectious sporoplasm into a host cell. The reactivity of mouse polyclonal antibodies raised against sporal proteins from two microsporidian species belonging to different genera (Glugea atherinae and Encephalitozoon cuniculi) was studied by western blotting and indirect immunofluorescence. Whole protein antisera provided a few cross-reactions relatable to some proteins of the spore envelope or polar tube. Ultrastructural immunocytochemistry with murine antibodies against protein bands separated by sodium dodecylsulphate polyacrylamide gel electrophoresis allowed the assignment of several proteins to the polar tube (34, 75 and 170 kDa in Glugea, 35, 55 and 150 kDa in Encephalitozoon). Antigenic similarities were detected for the Glugea 34 kDa and Encephalitozoon 35 kDa polar tube proteins. Species-specific proteins were shown to be located in either the lamellar polaroplast of Glugea or the spore envelope of Encephalitozoon.
微孢子虫可形成带有独特侵入装置的小孢子，该侵入装置具有一根长极管，极管的挤出可使感染性孢子质进入宿主细胞。通过蛋白质印迹法和间接免疫荧光法研究了针对来自不同属的两种微孢子虫（艾氏格留虫和兔脑炎微孢子虫）的孢子蛋白产生的小鼠多克隆抗体的反应性。全蛋白抗血清产生了一些与孢子包膜或极管的某些蛋白质相关的交叉反应。用抗十二烷基硫酸钠聚丙烯酰胺凝胶电泳分离的蛋白条带的鼠抗体进行超微结构免疫细胞化学分析，可将几种蛋白质定位到极管（格留虫中为34、75和170 kDa，脑炎微孢子虫中为35、55和150 kDa）。检测到格留虫34 kDa和脑炎微孢子虫35 kDa极管蛋白的抗原相似性。种特异性蛋白显示位于格留虫的层状极质体或脑炎微孢子虫的孢子包膜中。
