Partel C D, Rossi C L
Department of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas, São Paulo, Brazil.
Immunol Invest. 1998 Jan-Feb;27(1-2):89-96. doi: 10.3109/08820139809070892.
A rapid, quantitative enzyme-linked immunosorbent assay (ELISA) for the immunodiagnosis of Chagas' disease is described. The ELISA was standardized with the Falcon assay screening test (F.A.S.T.) system using a purified Trypanosoma cruzi epimastigote fraction (PEF) obtained by ion exchange chromatography. The ELISA technique and an indirect immunofluorescence (IIF) test were used to search for T.cruzi--specific IgG antibodies in 73 patients with chronic Chagas' disease, 58 patients with heterologous infections and 20 healthy controls. The ELISA exhibited 98.6% sensitivity and 98.7% specificity, whereas the sensitivity and specificity of the IIF test were 94.5% and 96.2%, respectively. The excellent performance with regard to sensitivity and specificity, as well as the short assay time make the F.A.S.T. system--based ELISA with PEF a potentially useful tool in screening for antibodies against T. cruzi.
本文描述了一种用于恰加斯病免疫诊断的快速定量酶联免疫吸附测定（ELISA）。该ELISA使用通过离子交换色谱法获得的纯化克氏锥虫上鞭毛体部分（PEF），以Falcon检测筛选试验（F.A.S.T.）系统进行标准化。采用ELISA技术和间接免疫荧光（IIF）试验，在73例慢性恰加斯病患者、58例异源感染患者和20例健康对照中检测克氏锥虫特异性IgG抗体。ELISA的敏感性为98.6%，特异性为98.7%，而IIF试验的敏感性和特异性分别为94.5%和96.2%。基于F.A.S.T.系统的ELISA结合PEF在敏感性和特异性方面表现出色，且检测时间短，使其成为筛查抗克氏锥虫抗体的潜在有用工具。
