Gallenga P E, Mastropasqua L, Carpineto P, Ciancaglini M, Falconio G, Catamo G, Costagliola C, Piccolomini R
Institute of Ophthalmology, G. D'Annunzio University, Chieti, Italy.
Ophthalmologica. 1998;212(3):184-7. doi: 10.1159/000027274.
The aim of our study was to verify the in vitro growth of Staphylococcus epidermidis in various dilutions of some viscoelastic substances containing hyaluronic acid (Healon and Healon GV, IAL, Biolon). Serial twofold dilutions of each sterile viscoelastic substance, prepared so as to obtain a final concentration ranging from 50 to 0.78% of the product in sterile saline solution (0.85% NaCl), were taken out with a pipette that delivered 1.0 ml/tube. One hundred microliters of the S. epidermidis inocula, used for the evaluation of the positive control of the test organism, was dispensed into each tube. After 24 h of aerobic incubation at 37 degrees C, 100 microl of sample was taken out from each tube and plated into the specific medium for the growth of the test organism. After 24 h of incubation at 37 degrees C, these agar plates were examined and the colony-forming unit count of the test organism was compared to the corresponding total colony count, acting as a positive control, in order to determine the quantitative variation of the test organism grown in the presence of the viscoelastic compounds. For the lowest dilutions (from 1:2 to 1:8) statistically significant bacterial growth was detected in all tested viscoelastic substances. For the highest dilutions (1:64 and 1:128) Biolon and Healon GV showed a significant inhibition of S. epidermidis growth. A significant inhibition was also observed in the highest dilution (1:128) of Healon. In every dilution of IAL a statistically significant increase in bacterial growth was observed. It remains to be carefully considered whether S. epidermidis, accidentally penetrating the eye via the intraocular lens, could find a culture medium in a small amount of sodium hyaluronate left in the capsular bag behind the optic.
我们研究的目的是验证表皮葡萄球菌在含透明质酸的某些粘弹性物质（Healon和Healon GV、IAL、Biolon）的不同稀释液中的体外生长情况。对每种无菌粘弹性物质进行系列两倍稀释，使其在无菌盐溶液（0.85%NaCl）中的最终浓度范围为产品的50%至0.78%，用每管输送1.0 ml的移液管取出。将用于测试生物体阳性对照评估的100微升表皮葡萄球菌接种物加入每管。在37℃需氧培养24小时后，从每管中取出100微升样品，接种到用于测试生物体生长的特定培养基中。在37℃培养24小时后，检查这些琼脂平板，并将测试生物体的菌落形成单位计数与作为阳性对照的相应总菌落计数进行比较，以确定在粘弹性化合物存在下生长的测试生物体的定量变化。对于最低稀释度（从1:2到1:8），在所有测试的粘弹性物质中均检测到具有统计学意义的细菌生长。对于最高稀释度（1:64和1:128），Biolon和Healon GV显示出对表皮葡萄球菌生长的显著抑制。在Healon的最高稀释度（1:128）中也观察到显著抑制。在IAL的每个稀释度中，均观察到细菌生长有统计学意义的增加。仍需仔细考虑，通过人工晶状体意外进入眼睛的表皮葡萄球菌是否能在晶状体后囊袋中残留的少量透明质酸钠中找到培养基。
