枯草芽孢杆菌色氨酸转运RNA（Trp）对乳白UGA密码子通读率的影响。

Effect of B. subtilis TRNA(Trp) on readthrough rate at an opal UGA codon.

作者信息

Matsugi J, Murao K, Ishikura H

机构信息

Laboratory of Chemistry, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi-machi, Tochigi 329-0498, Japan.

出版信息

J Biochem. 1998 May;123(5):853-8. doi: 10.1093/oxfordjournals.jbchem.a022015.

DOI:10.1093/oxfordjournals.jbchem.a022015

PMID:9562616

Abstract

Bacillus subtilis has been thought to have a high readthrough rate at the UGA stop codon because no opal suppressor tRNA has been isolated so far [Lovett et al. (1991) J. Bacteriol. 173, 1810-1812]. To examine whether a tRNATrp which we have characterized [Matsugi et al. (1992) Nucleic Acids Res. 20, 3514] has the ability to read the UGA codon, in vitro translation was performed with a synthetic mRNA containing a test codon, UGA, UAG, UAA, or UGG, in a reading frame. Addition of Trp-tRNATrp to the system significantly increased the readthrough rate only in the case of UGA. This suggests that this tRNATrp has a dual recognition pattern in B. subtilis, i.e., for the canonical tryptophan codon and for readthrough at the UGA stop codon.

摘要

由于至今尚未分离出乳白抑制tRNA，人们一直认为枯草芽孢杆菌在UGA终止密码子处具有较高的通读率[洛维特等人（1991年），《细菌学杂志》173卷，第1810 - 1812页]。为了检测我们已鉴定的tRNATrp[松木等人（1992年），《核酸研究》20卷，第3514页]是否具有读取UGA密码子的能力，我们使用了一个在阅读框中含有测试密码子UGA、UAG、UAA或UGG的合成mRNA进行体外翻译。仅在UGA的情况下，向系统中添加色氨酸tRNATrp会显著提高通读率。这表明这种tRNATrp在枯草芽孢杆菌中具有双重识别模式，即对典型的色氨酸密码子以及对UGA终止密码子的通读。