Pickering J W, Forghani B, Shell G R, Wu L
Gull Laboratories, Salt Lake City, UT 84117, USA.
Clin Diagn Virol. 1998 Jan;9(1):57-63. doi: 10.1016/s0928-0197(97)10004-6.
Diagnosis of acute and past infection with parvovirus B19 is based on detection of IgM and IgG antibodies.
To evaluate two commercial recombinant antigen-based enzyme immunoassay (EIA) test kits for detection of IgM and IgG antibodies to parvovirus B19 and to compare the commercial EIAs to in-house EIA test procedures.
A panel of 121 sera was used to compare the three IgM EIAs. The panel included 84 sera submitted for parvovirus B19 testing and 37 sera that were IgM positive for other viral pathogens. The same serum panel plus an additional 14 sera submitted for B19 testing was used to compare the three IgG EIAs. The commercial EIAs were performed according to manufacturers' instructions. Using the in-house EIA test procedures as the reference, sensitivity and specificity for each of the commercial EIAs was determined.
The commercial B19 IgM EIAs showed agreements of 95.0 and 93.4% to the in-house IgM EIA. Compared to the in-house B19 IgM EIA, the commercial B19 IgM EIAs were 97.4 and 97.5% sensitive, respectively. Specificities were 93.5 and 91.4%, respectively. Sensitivities for the commercial IgG EIAs, compared to in-house IgG EIA, were 88.0 and 85.2%, respectively, and specificities were 94.1 and 98.0%.
We found that the commercial parvovirus B19 IgM and IgG EIAs are comparable to standard in-house EIAs and are suitable for testing for B19 antibodies in human sera.
细小病毒B19急性感染及既往感染的诊断基于IgM和IgG抗体的检测。
评估两种基于重组抗原的商业酶免疫分析（EIA）检测试剂盒用于检测细小病毒B19的IgM和IgG抗体，并将商业EIA与内部EIA检测程序进行比较。
使用一组121份血清比较三种IgM EIA。该组包括84份提交用于细小病毒B19检测的血清和37份对其他病毒病原体IgM呈阳性的血清。使用相同的血清组加上另外14份提交用于B19检测的血清比较三种IgG EIA。商业EIA按照制造商的说明进行。以内部EIA检测程序作为参考，确定每种商业EIA的敏感性和特异性。
商业B19 IgM EIA与内部IgM EIA的一致性分别为95.0%和93.4%。与内部B19 IgM EIA相比，商业B19 IgM EIA的敏感性分别为97.4%和97.5%。特异性分别为93.5%和91.4%。与内部IgG EIA相比，商业IgG EIA的敏感性分别为88.0%和85.2%，特异性分别为94.1%和98.0%。
我们发现商业细小病毒B19 IgM和IgG EIA与标准的内部EIA相当，适用于检测人血清中的B19抗体。
