七种用于检测细小病毒B19特异性IgM的商业检测方法的比较。

Comparison of seven commercial tests for the detection of parvovirus B19-specific IgM.

作者信息

Schwarz T F, Jäger G, Gilch S

机构信息

Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University, Munich, Germany.

出版信息

Zentralbl Bakteriol. 1997 Apr;285(4):525-30. doi: 10.1016/s0934-8840(97)80114-4.

DOI:10.1016/s0934-8840(97)80114-4

PMID:9144914

Abstract

Several enzyme immunoassays (EIA) for the detection of parvovirus B19 IgM (anti-B19 IgM) are now commercially available. In this study, seven commercial EIAs (Biotrin, DAKO, Viramed, Viratech, R-Biopharm, Mast) were compared with an in-house EIA (MvP-EIA) using native viral B19 particles and the reference IgM radioimmunoassay (MACRIA). A total of 88 sera were tested. Results agreed in 39/88 (44.3%) sera, whereas 47/88 (53.4%) were discrepant and 2/88 (2.3%) gave an equivocal result. Assay sensitivity ranged from 70.3 to 100% and specificity, from 75.9 to 100%. The best results were obtained with two EIAs (Biotrin, DAKO) using baculovirus-expressed B19 proteins as antigen. This study has shown that baculovirus-expressed B19 antibody tests are suitable tools for detecting anti-B19 IgM.

摘要

目前有几种用于检测细小病毒B19 IgM（抗B19 IgM）的酶免疫测定法（EIA）在市场上销售。在本研究中，使用天然病毒B19颗粒和参考IgM放射免疫测定法（MACRIA），将七种商业EIA（Biotrin、DAKO、Viramed、Viratech、R-Biopharm、Mast）与一种内部EIA（MvP-EIA）进行了比较。共检测了88份血清。结果在39/88（44.3%）的血清中一致，而47/88（53.4%）存在差异，2/88（2.3%）给出了模棱两可的结果。检测灵敏度范围为70.3%至100%，特异性范围为75.9%至100%。使用杆状病毒表达的B19蛋白作为抗原的两种EIA（Biotrin、DAKO）获得了最佳结果。本研究表明，杆状病毒表达的B19抗体检测是检测抗B19 IgM的合适工具。