Hindman R, Tronic B, Bartlett R
J Clin Microbiol. 1976 Jul;4(1):102-3. doi: 10.1128/jcm.4.1.102-103.1976.
One hundred random urine specimens which were submitted for culture were planted using a calibrated loop within 2 h of collection and 2 and 4 h later after standing at room temperature. Colonies were counted after an 18- to 24-h incubation at 37 degrees C. Fifteen of the specimens demonstrated increases in counts exceeding 1 X log10; four increased from less than 10(5)/ml to greater than 10(5)/ml (three by the 4-h culture and a fourth by the 6-h culture), supporting the concept that delays of greater than 2 h in inoculating cultures may produce results which could cause errors in diagnosis.
将100份用于培养的随机尿液标本在采集后2小时内、室温放置2小时和4小时后，用校准接种环接种。在37℃孵育18至24小时后计数菌落。15份标本的菌落计数增加超过1×log10；4份标本的菌落数从小于10⁵/ml增加到大于10⁵/ml（3份在4小时培养时增加，第4份在6小时培养时增加），这支持了一个观点，即培养接种延迟超过2小时可能产生导致诊断错误的结果。
