Natah S S, Häyrinen-Immonen R, Hietanen J, Malmström M, Konttinen Y T
Department of Medicine, Helsinki University Central Hospital, Finland.
J Oral Pathol Med. 1998 Mar;27(3):124-9. doi: 10.1111/j.1600-0714.1998.tb01927.x.
Previous studies on the frequency of mast cells (MCs) in recurrent aphthous ulcers (RAU) have yielded conflicting results. Monoclonal antibodies specific for tryptase (AA1) and anti-IgE (polyclonal antibody) were used to identify density and distribution of MCs in an immunohistochemical study of RAU (n=15), induced oral traumatic ulcers (TUs) (n=9), and control clinically healthy oral mucosa (n=15). Results were quantified by means of a VIDAS image analyzer. In all sections studied, IgE-positive cells showed similar frequency and distribution to tryptase-positive MCs. In RAU lesions, numerous tryptase-positive MCs were found in the sub-epithelial lamina propria, but MC numbers in the epithelium were low and present only in some RAU biopsies. MCs were also more numerous in RAU-inflammatory infiltrates (118+/-31 cells/mm2) than those seen in TU-inflammatory infiltrates (75+/-18 cells/mm2, P<0.001). MC activation/degranulation, as judged by diffuse extracellular tryptase staining, was a common feature within RAU-inflammatory infiltrates and at RAU-inflammatory infiltrates-connective tissue interfaces, which were often associated with connective tissue disruption. MC counts in the RAU connective tissue, lateral to the inflammatory infiltrates, were significantly greater than in the connective tissue of TUs and of control biopsies (124+/-36 vs 73+/-13 vs 69+/-21 cells/mm2, respectively; P<0.001). Overall, MCs were significantly increased in aphthae (116+/-26 cells/mm2) compared with TU lesions (72+/-11 cells/mm2, P<0.001) and controls (71+/-16 cells/mm2, P<0.001). In conclusion, MC numbers are increased in a typical topographical pattern, and the local MCs show signs of activation/degranulation suggesting active involvement of this cell type in RAU pathogenesis.
先前关于复发性阿弗他溃疡（RAU）中肥大细胞（MCs）频率的研究结果相互矛盾。在一项对RAU（n = 15）、诱发性口腔创伤性溃疡（TUs）（n = 9）和临床健康口腔黏膜对照（n = 15）的免疫组织化学研究中，使用了对类胰蛋白酶特异的单克隆抗体（AA1）和抗IgE（多克隆抗体）来鉴定MCs的密度和分布。结果通过VIDAS图像分析仪进行定量分析。在所有研究的切片中，IgE阳性细胞显示出与类胰蛋白酶阳性MCs相似的频率和分布。在RAU病变中，上皮下固有层发现大量类胰蛋白酶阳性MCs，但上皮中的MC数量较少，且仅在部分RAU活检组织中存在。RAU炎症浸润中的MCs也比TU炎症浸润中的更多（118±31个细胞/mm²）（TU炎症浸润中为75±18个细胞/mm²，P<0.001）。通过弥漫性细胞外类胰蛋白酶染色判断，MC激活/脱颗粒是RAU炎症浸润内以及RAU炎症浸润-结缔组织界面的常见特征，这些界面常伴有结缔组织破坏。炎症浸润旁RAU结缔组织中的MC计数显著高于TU和对照活检组织的结缔组织（分别为124±36、73±13和69±21个细胞/mm²；P<0.001）。总体而言，与TU病变（72±11个细胞/mm²，P<0.001）和对照（71±16个细胞/mm²，P<0.001）相比，口疮中的MCs显著增加（116±26个细胞/mm²）。总之，MC数量以典型的拓扑模式增加，局部MCs显示出激活/脱颗粒迹象，提示该细胞类型积极参与RAU的发病机制。
