Bischoff S C, Wedemeyer J, Herrmann A, Meier P N, Trautwein C, Cetin Y, Maschek H, Stolte M, Gebel M, Manns M P
Department of Gastroenterology and Hepatology, Medical School of Hannover, Germany.
Histopathology. 1996 Jan;28(1):1-13. doi: 10.1046/j.1365-2559.1996.262309.x.
Previous studies on the frequency of intestinal mast cells and eosinophils in patients with inflammatory bowel disease yielded conflicting results. In the present morphometric study, we quantified mast cells and eosinophils in the lamina propria by histological and immunohistochemical methods in 64 patients suffering from Crohn's disease (33 cases) or ulcerative colitis (31 cases), and in 29 controls. Histological data from 206 biopsies were related to the presence of mucosal inflammation and clinical parameters. The number of eosinophils was increased in patients with inflammatory bowel conditions (mean +/- SE: 331 +/- 44/mm2) as compared to controls (258 +/- 27/mm2), and was dependent on disease activity and drug treatment. Mean mast cell numbers did not differ between patients and controls. However, a reduced mast cell number was found in toluidine blue-stained sections of actively inflamed tissue areas (143 +/- 16/mm2, versus 206 +/- 18/mm2 in non-inflamed tissue). Immunohistochemical studies using antibodies against the granule proteins tryptase and chymase suggest that this decrease in mast cell numbers is due to mast cell degranulation. The present data show that the number of intestinal mast cells and eosinophils is altered in patients with inflammatory bowel diseases, suggesting that both cell types are involved in the pathogenesis of chronic intestinal inflammation.
先前关于炎症性肠病患者肠道肥大细胞和嗜酸性粒细胞频率的研究结果相互矛盾。在本形态计量学研究中，我们采用组织学和免疫组织化学方法，对64例克罗恩病患者（33例）、溃疡性结肠炎患者（31例）以及29名对照者固有层中的肥大细胞和嗜酸性粒细胞进行了定量分析。来自206份活检标本的组织学数据与黏膜炎症的存在情况及临床参数相关。与对照组（258±27/mm²）相比，炎症性肠病患者的嗜酸性粒细胞数量增加（平均±标准误：331±44/mm²），且其数量取决于疾病活动度和药物治疗情况。患者与对照组的肥大细胞平均数量无差异。然而，在甲苯胺蓝染色的活跃炎症组织区域切片中发现肥大细胞数量减少（143±16/mm²，而非炎症组织为206±18/mm²）。使用针对颗粒蛋白类胰蛋白酶和糜酶的抗体进行的免疫组织化学研究表明，肥大细胞数量的减少是由于肥大细胞脱颗粒所致。目前的数据表明，炎症性肠病患者肠道肥大细胞和嗜酸性粒细胞数量发生改变，提示这两种细胞类型均参与了慢性肠道炎症的发病机制。
