Tseng S S, Weaver P L, Liu Y, Hitomi M, Tartakoff A M, Chang T H
Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA.
EMBO J. 1998 May 1;17(9):2651-62. doi: 10.1093/emboj/17.9.2651.
The DBP5 gene encodes a putative RNA helicase of unknown function in the yeast Saccharomyces cerevisiae. It is shown here that Dbp5p is an ATP-dependent RNA helicase required for polyadenylated [poly(A)+] RNA export. Surprisingly, Dbp5p is present predominantly, if not exclusively, in the cytoplasm, and is highly enriched around the nuclear envelope. This observation raises the possibility that Dbp5p may play a role in unloading or remodeling messenger RNA particles (mRNPs) upon arrival in the cytoplasm and in coupling mRNP export and translation. The functions of Dbp5p are likely to be conserved, since its potential homologues can be found in a variety of eukaryotic cells.
DBP5基因编码一种功能未知的假定RNA解旋酶，存在于酿酒酵母中。本文表明，Dbp5p是一种依赖ATP的RNA解旋酶，是聚腺苷酸化[poly(A)+]RNA输出所必需的。令人惊讶的是，Dbp5p主要（如果不是唯一的话）存在于细胞质中，并且在核膜周围高度富集。这一观察结果提出了一种可能性，即Dbp5p可能在信使RNA颗粒（mRNP）到达细胞质后卸载或重塑过程中发挥作用，并在mRNP输出与翻译的偶联中发挥作用。Dbp5p的功能可能是保守的，因为在多种真核细胞中都能找到其潜在的同源物。
