Waner T, Naveh A, Ben Meir N S, Babichev Z, Carmichael L E
Israel Institute for Biological Research, Ness Ziona, Israel.
Vet J. 1998 Mar;155(2):171-5. doi: 10.1016/s1090-0233(98)80013-0.
To study the response to vaccination, an enzyme-linked immunosorbant assay (ELISA) immunoblot method was developed and tested to assay canine distemper virus (CDV) IgG antibody in puppies and compared to a standard virus neutralization (VN) test (r2 = 0.748). Ten litters of four puppies each were used in a vaccination study. Seventy-six percent of vaccinated puppies immunized with a modified live vaccine were successfully protected against CDV at 6 weeks of age. One puppy remained seronegative after vaccination at 6 and 9 weeks of age. This is the first report of vaccination responses of puppies to CDV using an in-clinic test kit based on solid-phase immunoassay technology.
为研究疫苗接种反应，开发并测试了一种酶联免疫吸附测定（ELISA）免疫印迹法，用于检测幼犬体内的犬瘟热病毒（CDV）IgG抗体，并与标准病毒中和（VN）试验进行比较（r2 = 0.748）。在一项疫苗接种研究中使用了十窝幼犬，每窝四只。用改良活疫苗免疫的幼犬中，76%在6周龄时成功获得了针对CDV的保护。有一只幼犬在6周龄和9周龄接种疫苗后仍呈血清阴性。这是首次使用基于固相免疫测定技术的临床检测试剂盒报告幼犬对CDV的疫苗接种反应。
