Burriel A R, Scott M
Royal Veterinary College, Department of Farm Animal and Equine Medicine and Surgery, Hertfordshire, UK.
Vet J. 1998 Mar;155(2):183-8. doi: 10.1016/s1090-0233(98)80017-8.
Thirty reference strains of staphylococci, 76 strains isolated from confirmed cases of subclinical mastitis and 10 strains from the teat tip skin and the mouth of sucking lambs were assigned to species using four biochemical methods. These were the commercially available micromethods API Staph and Staph-Zym; the specialized laboratory method Rosco Set and Reactions in Standard Laboratory Culture Media (SLCM). The Rosco Set assigned species to all the strains, the SLCM to 96.5%, the Staph-zym to 86.1% and the API Staph to 81.4%. API Staph and the SLCM favoured the 'aureus' group with the 'epidermidis' second, while the reverse was true for the other two methods. All the identification methods assigned the same species to only 29% of the tested isolates, causing considerable variation in the number of species identified within each group. This variation indicates that reported species prevalence from cases of ovine mastitis should be viewed with caution.
使用四种生化方法将30株葡萄球菌参考菌株、76株从亚临床乳腺炎确诊病例中分离出的菌株以及10株从吮乳羔羊的乳头尖端皮肤和口腔中分离出的菌株进行菌种鉴定。这些方法包括市售的微量方法API Staph和Staph-Zym；专门的实验室方法Rosco Set以及标准实验室培养基（SLCM）中的反应。Rosco Set能对所有菌株进行菌种鉴定，SLCM能鉴定96.5%的菌株，Staph-zym能鉴定86.1%的菌株，API Staph能鉴定81.4%的菌株。API Staph和SLCM倾向于将菌株鉴定为“金黄色葡萄球菌”组，其次是“表皮葡萄球菌”组，而另外两种方法的结果则相反。所有鉴定方法仅对29%的测试分离株鉴定出相同的菌种，导致每组中鉴定出的菌种数量存在相当大的差异。这种差异表明，对于绵羊乳腺炎病例中报告的菌种流行情况应谨慎看待。
