de Mora F, Williams C M, Frenette P S, Wagner D D, Hynes R O, Galli S J
Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA.
Lab Invest. 1998 Apr;78(4):497-505.
Many studies, in both experimental animal and human systems, have indicated that P- and/or E-selectins may contribute importantly to the leukocyte recruitment that occurs in association with mast cell-dependent inflammatory responses. We used mice that genetically lack P-selectin (P -/-), E-selectin (E -/-), or both selectins (P/E -/-) to investigate the possible roles of these selectins in the IgE- and mast cell-dependent recruitment of neutrophils to the skin of mice. We found that a lack of either or both selectins had little or no effect on the extent of mast cell degranulation or the tissue swelling associated with these reactions. Moreover, a lack of either P- or E-selectin alone did not reduce the neutrophil infiltration at the reaction sites. However, mice lacking both P- and E-selectins exhibited an almost complete ablation of IgE- and mast cell-dependent neutrophil recruitment. These findings show that P- and E-selectins can express overlapping functions in leukocyte recruitment associated with IgE- and mast cell-dependent cutaneous late-phase reactions in mouse skin, and that a lack of both selectins results in a virtual elimination of IgE-dependent leukocyte recruitment.
许多在实验动物和人体系统中进行的研究表明，P选择素和/或E选择素可能在与肥大细胞依赖性炎症反应相关的白细胞募集中起重要作用。我们使用了基因敲除P选择素（P-/-）、E选择素（E-/-）或同时敲除这两种选择素（P/E-/-）的小鼠，来研究这些选择素在IgE和肥大细胞依赖性中性粒细胞向小鼠皮肤募集中的可能作用。我们发现，缺乏其中一种或两种选择素对肥大细胞脱颗粒程度或与这些反应相关的组织肿胀几乎没有影响。此外，单独缺乏P选择素或E选择素并不会减少反应部位的中性粒细胞浸润。然而，同时缺乏P选择素和E选择素的小鼠，IgE和肥大细胞依赖性中性粒细胞募集几乎完全消失。这些发现表明，P选择素和E选择素在与小鼠皮肤IgE和肥大细胞依赖性皮肤迟发反应相关的白细胞募集中可发挥重叠功能，并且同时缺乏这两种选择素会导致IgE依赖性白细胞募集几乎完全消除。
