Chakraborty P, Chowdhury I, Gupta-Bhattacharya S, Roy I, Chatterjee S, Chanda S
Department of Botany, Bose Institute, Calcutta, India.
Ann Allergy Asthma Immunol. 1998 Apr;80(4):311-7. doi: 10.1016/S1081-1206(10)62975-4.
The pollen grains of Arecaceae have been proved to be a very common aeroallergen in India. Except for the pollen grains of Cocos nucifera, detailed information about the allergenicity of other dominant Indian palm pollen is not available.
To explore the aerobiologic and allergenic significance of the pollen of palmyra palm (Borassus flabellifer Linn.), one of the important and common palm trees in India, with a view to isolate and purify its major allergenic components.
The aerobiologic survey was performed continuously using Burkard volumetric and Rotorod samplers from July, 1994 to June, 1996 in a northern suburb of greater Calcutta. The allergenic potential of the pollen extract and different fractions were studied by skin prick tests (SPT). ELISA and ELISA inhibition experiments were performed with individual and pooled patient sera to detect the specific IgE level. By 2-step ammonium sulphate fractionation and gel filtration study, the allergic fraction Fr.IIA1 was isolated. Its molecular weight was determined by SDS-PAGE and homogeneity was confirmed by rocket and crossed immunoelectrophoresis using rabbit antisera.
Pollen grains of B. flabellifer were found to be present in air from February to May and June contributing more than 7% of the total airborne pollen load of the study area. The pollen elicited maximum concentration at 19 to 20 hours in the evening and at a height of 4 m from ground level. Skin prick testing of 455 respiratory allergic patients with whole pollen extract exhibited 31.64% positive response. After 2-step ammonium sulphate fraction and gel filtration, a fraction (Fr.IIA1) having remarkable allergenicity was isolated. In native and SDS-PAGE, it was found as a single protein component of 90 kD and as homogeneous in RIE and CIE. The soluble protein-carbohydrate analysis indicated the probability of the component to be a glycoprotein.
The aerobiologic, clinical, and immunochemical, studies demonstrated that the pollen of B. flabellifer is a dominant aeroallergen to cause respiratory trouble in the area where these trees grow. The isolated 90-kD component is one of the major allergens present in the pollen extract.
棕榈科植物的花粉粒已被证明是印度一种非常常见的气传变应原。除了椰子的花粉粒外，关于印度其他主要棕榈花粉变应原性的详细信息尚无报道。
探讨印度重要且常见的棕榈树之一扇叶棕榈（Borassus flabellifer Linn.）花粉的空气生物学及变应原学意义，以期分离和纯化其主要变应原成分。
1994年7月至1996年6月，在加尔各答市北部郊区使用伯卡德容量采样器和旋转杆采样器持续进行空气生物学调查。通过皮肤点刺试验（SPT）研究花粉提取物及不同组分的变应原潜力。用个体患者血清和混合患者血清进行ELISA及ELISA抑制试验，以检测特异性IgE水平。通过两步硫酸铵分级分离和凝胶过滤研究，分离出变应性组分Fr.IIA1。用SDS-PAGE测定其分子量，并用兔抗血清通过火箭免疫电泳和交叉免疫电泳确认其均一性。
发现扇叶棕榈的花粉粒在2月至5月及6月存在于空气中，占研究区域空气传播花粉总量的7%以上。花粉在傍晚19至20时及离地面4米高度处浓度最高。用全花粉提取物对455例呼吸道过敏患者进行皮肤点刺试验显示阳性反应率为31.64%。经过两步硫酸铵分级分离和凝胶过滤后，分离出一种具有显著变应原性的组分（Fr.IIA1）。在天然及SDS-PAGE中，它表现为一条90 kD 的单一蛋白质条带，在火箭免疫电泳和交叉免疫电泳中显示均一性。可溶性蛋白质-碳水化合物分析表明该组分可能是一种糖蛋白。
空气生物学、临床及免疫化学研究表明，扇叶棕榈花粉是这些树木生长区域导致呼吸道疾病的主要气传变应原。分离出的90-kD组分是花粉提取物中的主要变应原之一。
