Chowdhury I, Chakraborty P, Gupta-Bhattacharya S, Chanda S
Department of Botany, Bose Institute, Calcutta, India.
Clin Exp Allergy. 1998 Aug;28(8):977-83. doi: 10.1046/j.1365-2222.1998.00364.x.
Palm pollen grains are predominant aeroallergens in the tropics including India. Evidence of allergenic crossreactivity had been reported from various parts of the world on different families, e.g. Poaceae, Asteraceae, etc. No such information is available about the palm pollen of tropical countries.
The present study was undertaken to find out the allergenic relationship, if any, in four common and important palm pollen in India.
A 2-year aerobiological survey was carried out at Madhyamgram situated at the suburban fringe of Calcutta Metropolis using Burkard volumetric sampler to know the seasonal variation of Areca catechu, Borassus flabellifer, Cocos nucifera and Phoenix sylvestris among others. Skin-prick tests (SPT) were performed with the relevant pollen extracts on the respiratory allergic patients. Sera from the subjects were tested directly by ELISA for estimating the allergen specific IgE. ELISA inhibitions and dot blotting were performed with pooled patients sera and four palm pollen extracts to detect the cross-reactivity.
Among 70 patients, Areca catechu exhibited the maximum percentage (48.5%) of positive responses followed by Cocos nucifera (45.7%), Phoenix sylvestris (42.85%) and Borassus flabellifer (38.5%). On an average, 30-50% of the patients showed positive skin reactions and enhanced specific IgE level to more than one palm pollen extract. Further evidence of allergenic crossreactivity among the four palm pollen grains were provided by dot blotting and ELISA inhibition studies. In ELISA inhibition, a distinct inhibition was obtained with comparable amounts of the pollen extracts.
The suburban aerobiological survey of Calcutta and SPT results confirmed that the relevant pollen types are significant contributors of aeroallergen load of the study area. ELISA inhibition studies with the pooled patients sera using antihuman IgE probe revealed the presence of shared allergenic components among the four palm pollen grains.
在包括印度在内的热带地区，棕榈花粉粒是主要的气传变应原。世界各地已报道了不同科（如禾本科、菊科等）之间变应原交叉反应性的证据。关于热带国家棕榈花粉的此类信息尚无报道。
本研究旨在查明印度四种常见且重要的棕榈花粉之间是否存在变应原关系。
在位于加尔各答市郊区边缘的马德亚姆格拉姆进行了为期两年的气传生物学调查，使用伯卡德容量采样器了解槟榔、扇叶棕榈、椰子和海枣等花粉的季节变化。对呼吸道过敏患者用相关花粉提取物进行皮肤点刺试验（SPT）。直接通过酶联免疫吸附测定（ELISA）检测受试者血清中的变应原特异性IgE。用患者混合血清和四种棕榈花粉提取物进行ELISA抑制试验和斑点印迹试验以检测交叉反应性。
在70名患者中，槟榔的阳性反应百分比最高（48.5%），其次是椰子（45.7%）、海枣（42.85%）和扇叶棕榈（38.5%）。平均而言，30%至50%的患者对一种以上的棕榈花粉提取物出现阳性皮肤反应且特异性IgE水平升高。斑点印迹试验和ELISA抑制试验进一步证明了四种棕榈花粉粒之间存在变应原交叉反应性。在ELISA抑制试验中，用等量的花粉提取物可获得明显的抑制作用。
加尔各答郊区的气传生物学调查和SPT结果证实，相关花粉类型是研究区域气传变应原负荷的重要贡献者。用抗人IgE探针进行的患者混合血清ELISA抑制试验表明，四种棕榈花粉粒之间存在共同的变应原成分。
