Nie L, Nicolau D P, Tessier P R, Kourea H P, Browner B D, Nightingale C H
Department of Orthopedic Surgery, Hartford Hospital, Connecticut 06102, USA.
J Orthop Res. 1998 Jan;16(1):76-9. doi: 10.1002/jor.1100160113.
This study was performed to investigate the potential utility of ofloxacin-impregnated bioabsorbable polymers for osteomyelitis therapy. Pseudomonas aeruginosa osteomyelitis was induced in 48 New Zealand White rabbits. Four weeks after infection, the animals were randomized to one of four treatment groups: drug-free polymer, ofloxacin polymer, systemic ofloxacin, or ofloxacin polymer plus systemic ofloxacin. Twenty-eight days later, radiographs were taken of the affected area, the animals were killed, and bone was obtained for histologic evaluation, culture, and determination of ofloxacin concentrations. The percentage of sterile bone cultures was 33, 83, 75, and 91 for the groups treated with drug-free polymer, ofloxacin polymer, systemic ofloxacin, and ofloxacin polymer plus systemic ofloxacin, respectively. When compared with the drug-free polymer, both the ofloxacin polymer and the ofloxacin polymer plus systemic ofloxacin significantly improved the rate of sterilization. The mean concentrations of the drug in bone for the groups treated with ofloxacin polymer, systemic ofloxacin, and ofloxacin polymer plus systemic ofloxacin were 34.9 (range: 2-160), 1.9 (range: 0.8-3), and 26.0 microg/g (range: 9-100 microg/g), respectively. These data suggest that the DL-lactide:glycolide polymer studied is a suitable vehicle for the delivery of high local concentrations of ofloxacin and that these concentrations result in eradication of the bacterial pathogen in this rabbit model.
本研究旨在探讨氧氟沙星浸渍的可生物吸收聚合物在骨髓炎治疗中的潜在效用。在48只新西兰白兔中诱发铜绿假单胞菌骨髓炎。感染四周后，将动物随机分为四个治疗组之一：不含药物的聚合物组、氧氟沙星聚合物组、全身用氧氟沙星组或氧氟沙星聚合物加全身用氧氟沙星组。28天后，对患部进行X光摄影，处死动物，获取骨骼进行组织学评估、培养及氧氟沙星浓度测定。不含药物的聚合物组、氧氟沙星聚合物组、全身用氧氟沙星组以及氧氟沙星聚合物加全身用氧氟沙星组无菌骨培养的百分比分别为33%、83%、75%和91%。与不含药物的聚合物组相比，氧氟沙星聚合物组和氧氟沙星聚合物加全身用氧氟沙星组均显著提高了杀菌率。氧氟沙星聚合物组、全身用氧氟沙星组以及氧氟沙星聚合物加全身用氧氟沙星组骨骼中药物的平均浓度分别为34.9（范围：2 - 160）、1.9（范围：0.8 - 3）和26.0微克/克（范围：9 - 100微克/克）。这些数据表明，所研究的DL-丙交酯：乙交酯聚合物是递送高局部浓度氧氟沙星的合适载体，且这些浓度可导致该兔模型中的细菌病原体被根除。
