Zhang K Z, Junnikkala S, Erlander M G, Guo H, Westberg J A, Meri S, Andersson L C
Department of Pathology, Haartman Institute, University of Helsinki, Finland.
Eur J Immunol. 1998 Apr;28(4):1189-96. doi: 10.1002/(SICI)1521-4141(199804)28:04<1189::AID-IMMU1189>3.0.CO;2-D.
We studied gene expression in relation to induced neural differentiation in a human neural crest-derived cell line, Paju. Messenger RNA isolated before and after treatment with phorbol 12-myristate 13-acetate was analyzed by differential display reverse transcription PCR. A strongly up-regulated expression of decay-accelerating factor (DAF, CD55) was found to parallel the induced neural sprouting while the expression of two other complement regulatory proteins (CD59/protectin, CD46/membrane cofactor protein) remained unaltered during neural differentiation. The increased membrane expression of DAF, which was also seen on neural processes and growth cones, conferred elevated resistance to complement-mediated lysis. Our findings suggest that in sprouting neurons DAF expression is up-regulated to provide additional complement resistance to pathfinding axons/dendrites invading new environment. It is also suggested that membrane expression of DAF may constitute a marker of growing and regenerating neurons.
我们研究了人神经嵴衍生细胞系Paju中与诱导神经分化相关的基因表达。用佛波酯12 - 肉豆蔻酸酯13 - 乙酸酯处理前后分离的信使核糖核酸通过差异显示逆转录聚合酶链反应进行分析。发现衰变加速因子（DAF，CD55）的强烈上调表达与诱导的神经芽生平行，而另外两种补体调节蛋白（CD59 /保护素，CD46 /膜辅因子蛋白）的表达在神经分化过程中保持不变。DAF在膜上的表达增加，这在神经突起和生长锥上也可见，赋予了对补体介导的溶解的更高抗性。我们的研究结果表明，在发芽神经元中，DAF表达上调，为侵入新环境的寻路轴突/树突提供额外的补体抗性。还表明，DAF的膜表达可能构成生长和再生神经元的标志物。
