编码丝氨酰 - tRNA合成酶的葡萄球菌基因在大肠杆菌中的分析及过表达

Analysis and overexpression in Escherichia coli of a staphylococcal gene encoding seryl-tRNA synthetase.

作者信息

Bausch N, Seignovert L, Beaulande M, Leberman R, Härtlein M

机构信息

European Molecular Biology Laboratory, Grenoble Outstation, B.P. 156, 38042 Grenoble Cedex, France.

出版信息

Biochim Biophys Acta. 1998 Apr 29;1397(2):169-74. doi: 10.1016/s0167-4781(98)00027-x.

DOI:10.1016/s0167-4781(98)00027-x

PMID:9565680

Abstract

We have sequenced and expressed in Escherichia coli the gene encoding the seryl-tRNA synthetase from the pathogenic bacterium Staphylococcus aureus. The overexpressed and purified recombinant enzyme was able to aminoacylate unfractionated tRNA from E. coli. Its activity was not affected by antibodies raised against and inhibiting the E. coli seryl-tRNA synthetase.

摘要

我们对致病性细菌金黄色葡萄球菌中编码丝氨酰 - tRNA合成酶的基因进行了测序，并在大肠杆菌中进行了表达。过量表达并纯化的重组酶能够将大肠杆菌中未分级的tRNA氨酰化。其活性不受针对大肠杆菌丝氨酰 - tRNA合成酶产生并抑制该酶的抗体的影响。