Härtlein M, Madern D, Leberman R
Nucleic Acids Res. 1987 Feb 11;15(3):1005-17. doi: 10.1093/nar/15.3.1005.
Seryl-tRNA synthetase is the gene product of the serS locus in Escherichia coli. Its gene has been cloned by complementation of a serS temperature sensitive mutant K28 with an E. coli gene bank DNA. The resulting clones overexpress seryl-tRNA synthetase by a factor greater than 50 and more than 6% of the total cellular protein corresponds to the enzyme. The DNA sequence of the complete coding region and the 5'- and 3' untranslated regions was determined. Protein sequence comparison of SerRS with all available aminoacyl-tRNA synthetase sequences revealed some regions of significant homology particularly with the isoleucyl- and phenylalanyl-tRNA synthetases from E. coli.
丝氨酰 - tRNA合成酶是大肠杆菌中serS基因座的基因产物。它的基因已通过用大肠杆菌基因文库DNA对serS温度敏感突变体K28进行互补克隆得到。所得克隆使丝氨酰 - tRNA合成酶的表达量超过50倍，并且超过6%的总细胞蛋白对应于该酶。测定了完整编码区以及5'和3'非翻译区的DNA序列。将丝氨酰 - tRNA合成酶（SerRS）与所有可用的氨酰 - tRNA合成酶序列进行蛋白质序列比较，发现了一些显著同源的区域，特别是与大肠杆菌的异亮氨酰 - tRNA合成酶和苯丙氨酰 - tRNA合成酶。
