Smiałowska M, Bajkowska M
Department od Neurobiology, Polish Academy od Sciences, Kraków, Poland.
Pol J Pharmacol. 1997 Sep-Oct;49(5):357-62.
Neuropeptide Y (NPY), a 36-amino acid peptide, is present in some hippocampal interneurons and nerve terminals and seems to modulate glutamatergic transmission in this structure. Earlier studies of some other authors showed an increase in NPY expression in the hippocampus during seizures and activation of ionotropic glutamate receptors. In the present study the effect of metabotropic glutamate receptor (mGluR) stimulation was investigated in rat hippocampus by immunohistochemical methods. It was found that (1S,3R)1-aminocyclopentane-1,3-dicarboxylic acid (1S,3R-ACPD), a selective agonist of mGluRs injected into the rat lateral ventricle (1 mumol/10 microliters) or hippocampus (0.1 mumol/1 microliter), 24 h before taking the brains for immunohistochemical studies, induced a significant increase in NPY-immunoreactivity in the hippocampus, especially in the hilar region. The obtained results indicate that mGluRs positively modulate the NPY content in the hippocampal neurons.
神经肽Y（NPY）是一种由36个氨基酸组成的肽，存在于一些海马中间神经元和神经末梢中，似乎能调节该结构中的谷氨酸能传递。其他一些作者早期的研究表明，癫痫发作期间海马中NPY表达增加以及离子型谷氨酸受体激活。在本研究中，采用免疫组织化学方法研究了代谢型谷氨酸受体（mGluR）刺激对大鼠海马的影响。结果发现，在取脑进行免疫组织化学研究前24小时，向大鼠侧脑室（1微摩尔/10微升）或海马（0.1微摩尔/1微升）注射mGluRs的选择性激动剂（1S，3R）1-氨基环戊烷-1，3-二羧酸（1S，3R-ACPD），可诱导海马中NPY免疫反应性显著增加，尤其是在门区。所得结果表明，mGluRs对海马神经元中的NPY含量具有正向调节作用。
