Abath F G, Xavier E M, Silva J D, Morais Júnior M A, Montenegro S M
Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães-FIOCRUZ, Recife, PE, Brasil.
Mem Inst Oswaldo Cruz. 1997 Sep-Oct;92(5):637-41. doi: 10.1590/s0074-02761997000500014.
Sm15 and Sm13 are recognized by antibodies from mice protectively vaccinated with tegumental membranes, suggesting a potential role in protective immunity. In order to raise antibodies for immunochemical investigations, the genes for these antigens were expressed in pGEX and pMal vectors so that comparisons could be made among different expression systems and different genes. The fusion proteins corresponding to several parts of the gene for the precursor of Sm15 failed in producing antibodies recognizing the parasite counterpart. On the other hand, antibodies raised against Sm13 MBP-fusion proteins recognized the 13 kDa tegumental protein. Thus the peculiarities of the gene of interest are important and the choice of the expression system must sometimes be decided on an empirical basis.
Sm15和Sm13可被用被膜抗原进行保护性免疫接种的小鼠产生的抗体识别，这表明它们在保护性免疫中可能发挥作用。为了制备用于免疫化学研究的抗体，这些抗原的基因在pGEX和pMal载体中表达，以便对不同的表达系统和不同的基因进行比较。与Sm15前体基因几个部分相对应的融合蛋白未能产生识别寄生虫对应物的抗体。另一方面，针对Sm13 MBP融合蛋白产生的抗体识别13 kDa的被膜蛋白。因此，目标基因的特性很重要，有时必须根据经验来决定表达系统的选择。
