Horie T, Nakamaru M, Masubuchi Y
Laboratory of Biopharmaceutics, Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Life Sci. 1998;62(15):1333-8. doi: 10.1016/s0024-3205(98)00067-8.
Oral administration of methotrexate (MTX) to mice causes the damage of small intestine. The permeability of poorly absorbable compound fluorescein isothiocyanate (FITC)-labeled dextran (average molecular weight, 4,400) through the small intestine was studied in vitro using everted segments of the small intestine. The permeability of FITC-dextran increased remarkably in the MTX-treated mice and oral administration of docosahexaenoic acid ethyl ester (DHA) protected the small intestine from the increase in the small intestinal permeability induced by the MTX treatment. The MTX treatment decreased retinol concentration in plasma of mice and the coadministration of DHA maintained its concentration to the level of control mice. The present study showed that DHA protected the small intestine of mice from the MTX-induced damage.
给小鼠口服甲氨蝶呤（MTX）会导致小肠损伤。使用小肠外翻段在体外研究了难吸收化合物异硫氰酸荧光素（FITC）标记的右旋糖酐（平均分子量4400）通过小肠的通透性。FITC - 右旋糖酐的通透性在MTX处理的小鼠中显著增加，而口服二十二碳六烯酸乙酯（DHA）可保护小肠免受MTX处理诱导的小肠通透性增加的影响。MTX处理降低了小鼠血浆中的视黄醇浓度，而DHA的共同给药将其浓度维持在对照小鼠的水平。本研究表明，DHA保护小鼠小肠免受MTX诱导的损伤。
