Department of Gastrointestinal Microbiology, German Institute of Human Nutrition Potsdam-Rehbruecke, Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, Germany.
Nutrients. 2018 May 28;10(6):685. doi: 10.3390/nu10060685.
Protocols for intestinal permeability measurements in mice using 4-kDa fluorescein isothiocyanate-conjugated (FITC) dextran differ considerably among laboratories on the blood-sampling time. To find the optimal point in time for blood sampling, we administered 4-kDa FITC dextran to C3H mice and monitored the marker in plasma over 8 h. We also determined gut-transit time using 70-kDa FITC dextran, which does not cross the intestinal epithelium. The 4-kDa FITC dextran concentration in plasma reached its maximum 45 min after administration. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC dextran measured in plasma 1 h after its oral application is a marker of small intestinal permeability.
在使用 4kDa 荧光素异硫氰酸酯标记（FITC）葡聚糖进行肠道通透性测量的方案中，不同实验室之间在采血时间上存在很大差异。为了找到采血的最佳时间点，我们给 C3H 小鼠施用了 4kDa FITC 葡聚糖，并在 8 小时内监测了血浆中的标记物。我们还使用不能穿过肠上皮的 70kDa FITC 葡聚糖来确定肠道转运时间。在给药后 45 分钟，血浆中 4kDa FITC 葡聚糖的浓度达到最大值。70kDa FITC 葡聚糖在给药后 15 分钟到达空肠，并在给药后 1 小时内通过整个小肠，这表明在口服应用后 1 小时测量的血浆中的 4kDa FITC 葡聚糖是小肠通透性的标志物。
