绘制20种抗补体受体1单克隆抗体的表位图谱。
Mapping epitopes for 20 monoclonal antibodies to CR1.
作者信息
Nickells M, Hauhart R, Krych M, Subramanian V B, Geoghegan-Barek K, Marsh H C, Atkinson J P
机构信息
Washington University School of Medicine, St Louis, MO 63110, USA.
出版信息
Clin Exp Immunol. 1998 Apr;112(1):27-33. doi: 10.1046/j.1365-2249.1998.00549.x.
Abstract
Complement receptor type one (CR1; CD35) binds and processes C3b and C4b opsonized immune complexes and regulates complement activation. We have characterized the epitopes of 13 previously reported and seven new MoAbs to human CR1. The MoAbs formed seven groups based on their reactivity with a panel of deletion forms of CR1. Seventeen of the MoAbs reacted with CR1 at more than one site, a consequence of its repetitive sequence. All five of the MoAbs recognizing epitopes in the nearly identical repeats 3, 10, and 17, as well as one MoAb which reacted with repeats 8 or 1/2 of 9 and 15 or 1/2 of 16, blocked cofactor activity for C3b. Knowledge of the repeats bearing the epitopes for these MoAbs should facilitate the further characterization of CR1.
摘要
一型补体受体（CR1；CD35）可结合并处理被C3b和C4b调理的免疫复合物，并调节补体激活。我们已对先前报道的13种及7种针对人CR1的新单克隆抗体的表位进行了表征。这些单克隆抗体根据其与一组CR1缺失形式的反应性形成了7组。其中17种单克隆抗体在多个位点与CR1发生反应，这是其重复序列的结果。所有5种识别几乎相同的重复序列3、10和17中表位的单克隆抗体，以及一种与重复序列8或9的1/2以及15或16的1/2发生反应的单克隆抗体，均阻断了C3b的辅因子活性。了解这些单克隆抗体表位所在的重复序列应有助于对CR1进行进一步表征。
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本文引用的文献
1
A soluble deletion mutant of the human complement receptor type 1, which lacks the C4b binding site, is a selective inhibitor of the alternative complement pathway.一种缺乏C4b结合位点的人补体受体1可溶性缺失突变体是替代补体途径的选择性抑制剂。
Eur J Immunol. 1996 Aug;26(8):1729-35. doi: 10.1002/eji.1830260810.
2
Analysis of the functional domains of complement receptor type 1 (C3b/C4b receptor; CD35) by substitution mutagenesis.通过置换诱变分析1型补体受体（C3b/C4b受体；CD35）的功能结构域。
J Biol Chem. 1994 May 6;269(18):13273-8.
3
Cross-linked bispecific monoclonal antibody heteropolymers facilitate the clearance of human IgM from the circulation of squirrel monkeys.交联双特异性单克隆抗体异聚物有助于从松鼠猴的循环系统中清除人IgM。
Eur J Immunol. 1994 Sep;24(9):2018-25. doi: 10.1002/eji.1830240913.
4
Identification of complement receptor type 1-related proteins on primate erythrocytes.灵长类红细胞上1型补体受体相关蛋白的鉴定。
J Immunol. 1995 Mar 15;154(6):2829-37.
5
Functional analysis of complement receptor 1 using a new monoclonal antibody, KuN241.
Hybridoma. 1995 Feb;14(1):29-35. doi: 10.1089/hyb.1995.14.29.
6
Human complement (C3b) receptors defined by a mouse monoclonal antibody.由小鼠单克隆抗体界定的人补体（C3b）受体。
Immunology. 1982 Apr;45(4):645-53.
7
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J Exp Med. 1981 May 1;153(5):1138-50. doi: 10.1084/jem.153.5.1138.
8
Primate erythrocyte-immune complex-clearing mechanism.灵长类动物红细胞免疫复合物清除机制。
J Clin Invest. 1983 Feb;71(2):236-47. doi: 10.1172/jci110764.
9
Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1).鉴定一种对膜补体受体1（CR1）具有特异性的抗单核细胞单克隆抗体。
Eur J Immunol. 1984 Mar;14(3):236-43. doi: 10.1002/eji.1830140307.
10
Mouse monoclonal antibodies to the human C3b receptor.针对人C3b受体的小鼠单克隆抗体。
Mol Immunol. 1985 May;22(5):531-9. doi: 10.1016/0161-5890(85)90176-2.
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