人胎肝中重排α重链基因的互补决定区3（CDR3）区域的特征分析
Characterization of the CDR3 region of rearranged alpha heavy chain genes in human fetal liver.
作者信息
Baskin B, Islam K B, Smith C I
机构信息
Department of Biosciences, NOVUM, Karolinska Institute, Huddinge, Sweden.
出版信息
Clin Exp Immunol. 1998 Apr;112(1):44-7. doi: 10.1046/j.1365-2249.1998.00547.x.
The human fetal liver is an early site for B cell development. Pre B cells are first detectable in human fetal life at 8 weeks of gestation, when the rearrangement of the mu heavy chain genes starts. In this study we characterize the CDR3 region of rearranged alpha heavy chain transcripts from four human fetal livers ranging from 8 to 11 weeks of gestation. Each fetal liver showed a limited number of variations in CDR3 sequences compared with adult peripheral blood mononuclear cells (PBMC). Sequence analysis of 91 clones demonstrated that there was no preference for the usage of a certain JH gene segment, whereas a preference for usage of DH family genes, DXP and DLR, was seen in most cases during early fetal life. This is the first study where rearranged alpha heavy chain genes in fetal liver have been characterized. Our data suggest that the usage of JH genes is random, while there is a preference for DH family genes in human fetal liver.
人类胎儿肝脏是B细胞发育的早期场所。前B细胞最早在妊娠8周的人类胎儿期被检测到，此时μ重链基因开始重排。在本研究中，我们对来自妊娠8至11周的四个人类胎儿肝脏中重排的α重链转录本的互补决定区3（CDR3）区域进行了特征分析。与成人外周血单个核细胞（PBMC）相比，每个胎儿肝脏的CDR3序列变化数量有限。对91个克隆的序列分析表明，在使用特定JH基因片段方面没有偏好，而在胎儿早期的大多数情况下，可见对DH家族基因DXP和DLR的使用偏好。这是首次对胎儿肝脏中重排的α重链基因进行特征分析的研究。我们的数据表明，JH基因的使用是随机的，而人类胎儿肝脏中对DH家族基因存在偏好。
Zotero 插件