Waine G J, Yang W, Ross A G, Li Y S, Sleigh A C, Kalinna B H, Scott J C, Mazzer D, Li Y, McManus D P
Molecular Parasitology Unit, The Queensland Institute of Medical Research, PO Royal Brisbane Hospital, Australia.
Clin Exp Immunol. 1998 Apr;112(1):69-73. doi: 10.1046/j.1365-2249.1998.00542.x.
Peripheral blood mononuclear cells (PBMC) from 117 individuals living on two islands in an area (Dongting Lake) endemic for schistosomiasis japonica in China, and 15 control individuals from a non-endemic area of China, were assessed for antigen-stimulated proliferation against five recombinant Schistosoma japonicum antigens of recognized interest in the development of immunity to schistosomiasis. Two recombinant antigens, paramyosin and 28-kD glutathione-S-transferase, stimulated cellular proliferation (stimulation index > or = 3.0) in 38.5% and 42.5% of subjects, respectively, a level similar to that induced by a soluble whole parasite extract (51.3%). In contrast, three other recombinant antigens tested--a fatty acid binding protein, 22-kD tegumental membrane-associated antigen, and glyceraldehyde-3-phosphate dehydrogenase--stimulated PBMC proliferation in only 3-8% of subjects. Moreover, we also identified a positive association between the degree of exposure, and cellular proliferation following stimulation with recombinant paramyosin or whole parasite extract. Highly significant differences in antigen-stimulated proliferation were also observed between the two islands, Niangashan and Qingshan. The whole parasite extract stimulated proliferation in 90% of subjects from Niangashan island compared with only 42.1% of subjects from Qingshan island (chi2 = 16.88, P = 0.00004), while glutathione-S-transferase stimulated proliferation in 77.3% of subjects from Niangashan island compared with only 34.7% of subjects from Qingshan island (chi2 = 13.09, P = 0.003). A similar, but not significant, trend was observed for paramyosin and the fatty-acid binding protein. The identification of differential cellular proliferative responses to specific schistosome antigens within an infected human population may have important practical implications for vaccine development against schistosomiasis japonica.
从中国日本血吸虫病流行区（洞庭湖）两个岛屿的117名居民以及来自中国非流行区的15名对照个体采集外周血单个核细胞（PBMC），评估其针对5种已确认对日本血吸虫病免疫发展有重要意义的重组日本血吸虫抗原的抗原刺激增殖情况。两种重组抗原，副肌球蛋白和28-kD谷胱甘肽-S-转移酶，分别刺激了38.5%和42.5%的受试者细胞增殖（刺激指数≥3.0），这一水平与可溶性全虫提取物诱导的水平相似（51.3%）。相比之下，另外三种测试的重组抗原——脂肪酸结合蛋白、22-kD皮层膜相关抗原和甘油醛-3-磷酸脱氢酶——仅刺激了3%-8%的受试者的PBMC增殖。此外，我们还发现接触程度与用重组副肌球蛋白或全虫提取物刺激后的细胞增殖之间存在正相关。在娘嘎山和青山两个岛屿之间，抗原刺激增殖也观察到了高度显著的差异。全虫提取物刺激了娘嘎山岛90%的受试者增殖，而青山岛只有42.1%的受试者增殖（χ2 = 16.88，P = 0.00004），而谷胱甘肽-S-转移酶刺激了娘嘎山岛77.3%的受试者增殖，而青山岛只有34.7%的受试者增殖（χ2 = 13.09，P = 0.003）。副肌球蛋白和脂肪酸结合蛋白也观察到了类似但不显著的趋势。在受感染人群中鉴定对特定血吸虫抗原的不同细胞增殖反应，可能对日本血吸虫病疫苗的开发具有重要的实际意义。
