Bluming A, Homer S, Khiroya R
J Lab Clin Med. 1976 Sep;88(3):417-22.
Diphenylhydantoin (DPH) in concentrations approximating therapeutic blood levels was found to inhibit mixed lymphocyte culture (MLC) proliferation as measured by uptake of 3H-thymidine when compared to alkaline diluent required to solubilize the DPH. Control cultures containing diluent alone manifested inhibition of 3H-thymidine uptake relative to non-diluent-containing cultures. Both reactor and stimulator cells were independently capable of effecting this inhibition after treatment with DPH. An inconsistent effect of DPH on lymphocyte reactivity to graded doses of phytohemagglutinin and to pokeweed mitogen was observed. A possible association between the effect of DPH on MLC reactivity and the phenomenon of dilantin-induced lymphadenopathy is suggested.
与溶解苯妥英钠所需的碱性稀释剂相比，发现苯妥英钠（DPH）在接近治疗血药浓度时可抑制混合淋巴细胞培养（MLC）增殖，这是通过3H-胸腺嘧啶核苷摄取来测量的。仅含稀释剂的对照培养物相对于不含稀释剂的培养物表现出3H-胸腺嘧啶核苷摄取的抑制。在用DPH处理后，反应细胞和刺激细胞均能独立产生这种抑制作用。观察到DPH对淋巴细胞对分级剂量的植物血凝素和商陆有丝分裂原的反应性有不一致的影响。提示了DPH对MLC反应性的影响与苯妥英钠诱导的淋巴结病现象之间可能存在关联。
