Grahm G, Bates G W
J Lab Clin Med. 1976 Sep;88(3):477-86.
An important aspect in the development and use of serum unsaturated iron-binding capacity (UIBC) procedures is the standardization of reference serum. Spectrophotometric titration of serum transferrin is the oldest of the UIBC methods; however, the literature on this subject indicates that the technique is not being fully exploited and describes a variety of suboptimal conditions. A spectrophotometric titration protocol is described which gives excellent reproducibility with fresh, frozen, lyophilized, and stored serum samples. An indirect UIBC method utilizing differential chelation and ultrafiltration is also described. In this method Fe3+ -NTA (nitrilotriacetic acid), in excess of the anticipated UIBC, is added to a serum sample supplemented with NaHC03. The addition of EDTA (ethelenediaminetetraacetic acid) allows the mobilization of iron from nonspecific macromolecular sites. The ultrafiltrate is analyzed for iron, and the UIBC is calculated. The results were validated via an ultrafiltration titration which was compared with a spectrophotometric titration. The precision of this method is excellent.
血清不饱和铁结合能力（UIBC）检测方法的开发与应用中一个重要方面是参考血清的标准化。血清转铁蛋白的分光光度滴定法是最古老的UIBC检测方法；然而，关于该主题的文献表明该技术尚未得到充分利用，并且描述了各种不理想的条件。本文描述了一种分光光度滴定方案，该方案对新鲜、冷冻、冻干和储存的血清样本具有出色的重现性。还描述了一种利用差异螯合和超滤的间接UIBC检测方法。在该方法中，将过量预期UIBC的Fe3+-NTA（次氮基三乙酸）添加到补充有NaHCO3的血清样本中。添加EDTA（乙二胺四乙酸）可使铁从非特异性大分子位点释放出来。对超滤液进行铁分析，并计算UIBC。通过与分光光度滴定法比较的超滤滴定法对结果进行了验证。该方法的精密度极佳。
