Kirkman H N, Lanier D C, Clemons E H, Sanderford J L
J Lab Clin Med. 1976 Sep;88(3):515-9.
A method is described for the enzymic estimation of galactose-1-phosphate (Gal-1-P) in blood which has been applied to filter paper and allowed to dry. The successful clinical management of patients with galactosemia depends upon exclusion of galactose from their diet. Earlier studies demonstrated that red cell Gal-1-P is a sensitive indicator of exposure of such patients to galactose. These earlier methods, however, required venipuncture, preparation of washed, packed red cells, and shipment of the sample in dry ice to a central laboratory. With the present method, capillary blood can be drawn by a nonphysician, applied to filter paper and mailed in a conventional envelope at ambient temperature. From this sample, the Gal-1-P content of the red cells can be determined, if the hematocrit is known. These conveniences should allow estimates of Gal-1-P at a frequency more appropirate for optimal dietary control.
本文描述了一种用于酶法测定血液中1-磷酸半乳糖（Gal-1-P）的方法，该方法已应用于滤纸并使其干燥。半乳糖血症患者的成功临床管理取决于从其饮食中排除半乳糖。早期研究表明，红细胞Gal-1-P是此类患者接触半乳糖的敏感指标。然而，这些早期方法需要静脉穿刺、制备洗涤过的压积红细胞，并将样品用干冰运送到中心实验室。使用本方法，非医生人员即可采集毛细血管血，将其应用于滤纸，并在室温下用普通信封邮寄。如果已知血细胞比容，则可从该样品中测定红细胞的Gal-1-P含量。这些便利条件应能以更适合最佳饮食控制的频率对Gal-1-P进行检测。
