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加州海兔产卵激素的生化分离与生理鉴定

Biochemical isolation and physiological identification of the egg-laying hormone in Aplysia californica.

作者信息

Arch S, Earley P, Smock T

出版信息

J Gen Physiol. 1976 Aug;68(2):197-210. doi: 10.1085/jgp.68.2.197.

DOI:10.1085/jgp.68.2.197
PMID:956770
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2228423/
Abstract

It has been determined that the bag cells of Aplysia californica produce two polypeptide species that comigrate on electrophoretic gels containing sodium dodecyl sulfate. By this separation procedure both species can be assigned a molecular weight of approximately 6,000. One of these molecules has an Rf of 0.65 on alkaline discontinuous electrophoresis gels, an isoelectric point at pH 4.8, a gel filtration molecular weight of approximately 12,000, and has no known biological function. The other does not enter alkaline disk gels, has an isoelectric point at approximately pH 9.3, shows a gel filtration molecular weight consistent with that determined by SDS gel electrophoresis, and is the egg-laying hormone.

摘要

现已确定，加州海兔的袋状细胞产生两种多肽，它们在含有十二烷基硫酸钠的电泳凝胶上迁移率相同。通过这种分离方法，这两种多肽的分子量都约为6000。其中一种分子在碱性不连续电泳凝胶上的Rf为0.65，等电点为pH 4.8，凝胶过滤分子量约为12000，且没有已知的生物学功能。另一种则不进入碱性圆盘凝胶，等电点约为pH 9.3，其凝胶过滤分子量与SDS凝胶电泳测定的结果一致，它就是产卵激素。

相似文献

1
Biochemical isolation and physiological identification of the egg-laying hormone in Aplysia californica.加州海兔产卵激素的生化分离与生理鉴定
J Gen Physiol. 1976 Aug;68(2):197-210. doi: 10.1085/jgp.68.2.197.
2
Purification and primary structure of the neuropeptide egg-laying hormone of Aplysia californica.加州海兔神经肽产卵激素的纯化及一级结构
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Neurosecretion of egg-laying hormone and other peptides from electrically active bag cell neurons of Aplysia.
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Precursor and product processing in the bag cell neurons of Aplysia californica.加州海兔袋状细胞神经元中的前体和产物加工
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Atrial gland cells synthesize a family of peptides that can induce egg laying in Aplysia.心房腺细胞合成了一类能诱导海兔产卵的肽。
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Isolation of an egg-laying hormone-binding protein from the gonad of Aplysia californica and its localization in oocytes.从加州海兔性腺中分离产卵激素结合蛋白及其在卵母细胞中的定位。
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Purification and primary structure of two neuroactive peptides that cause bag cell afterdischarge and egg-laying in Aplysia.两种引起海兔套膜细胞放电后效应及产卵的神经活性肽的纯化与一级结构
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Proteolytic processing in the biogenesis of the neurosecretory egg-laying hormone in Aplysia. 1. Precursors, intermediates, and products.海兔神经分泌性产卵激素生物合成中的蛋白水解加工。1. 前体、中间体和产物。
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引用本文的文献

1
Primary structure and neuronal effects of alpha-bag cell peptide, a second candidate neurotransmitter encoded by a single gene in bag cell neurons of Aplysia.α-袋细胞肽的一级结构和神经元效应，α-袋细胞肽是 Aplysia 袋细胞神经元中单个基因编码的第二种候选神经递质。
Proc Natl Acad Sci U S A. 1983 Sep;80(18):5753-7. doi: 10.1073/pnas.80.18.5753.
2
Isolation of an egg-laying hormone-binding protein from the gonad of Aplysia californica and its localization in oocytes.从加州海兔性腺中分离产卵激素结合蛋白及其在卵母细胞中的定位。
J Comp Physiol A. 1993 Oct;173(4):475-83. doi: 10.1007/BF00193520.
3
Atrial gland cells synthesize a family of peptides that can induce egg laying in Aplysia.心房腺细胞合成了一类能诱导海兔产卵的肽。
J Comp Physiol B. 1985;156(1):43-55. doi: 10.1007/BF00692925.
4
The bag cell neurons of Aplysia. A model for the study of the molecular mechanisms involved in the control of prolonged animal behaviors.海兔的袋状细胞神经元。一个用于研究控制动物长期行为所涉及分子机制的模型。
Mol Neurobiol. 1989 Winter;3(4):237-73. doi: 10.1007/BF02740607.
5
Cyclic AMP regulates processing of neuropeptide precursor in bag cell neurons of Aplysia.环磷酸腺苷调节海兔袋状细胞神经元中神经肽前体的加工过程。
J Mol Neurosci. 1990;2(2):61-70. doi: 10.1007/BF02876912.
6
Purification and primary structure of the neuropeptide egg-laying hormone of Aplysia californica.加州海兔神经肽产卵激素的纯化及一级结构
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6656-60. doi: 10.1073/pnas.76.12.6656.
7
Evidence for mediation of a neuronal interaction by a behaviorally active peptide.行为活性肽介导神经元相互作用的证据。
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5732-6. doi: 10.1073/pnas.75.11.5732.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Study of the dansylation reaction of amino acids, peptides and proteins.氨基酸、肽和蛋白质的丹磺酰化反应研究。
Eur J Biochem. 1969 Feb;7(4):463-70. doi: 10.1111/j.1432-1033.1969.tb19632.x.
3
Disc electrophoresis.圆盘电泳
J Chem Educ. 1969 Jan;46(1):41-5. doi: 10.1021/ed046p41.
4
Methods for fractionation and scintillation counting of radioisotope-labeled polyacrylamide gels.放射性同位素标记聚丙烯酰胺凝胶的分级分离和闪烁计数方法。
Anal Biochem. 1970 Nov;38(1):90-7. doi: 10.1016/0003-2697(70)90158-2.
5
Isoelectric focusing of proteins in the native and denatured states. Anomalous behaviour of plasma albumin.天然和变性状态下蛋白质的等电聚焦。血浆白蛋白的异常行为。
Biochem J. 1971 Mar;122(1):93-9. doi: 10.1042/bj1220093.
6
Biosynthesis of the egg-laying hormone (ELH) in the bag cell neurons of Aplysia californica.加州海兔袋状细胞神经元中产卵激素（ELH）的生物合成。
J Gen Physiol. 1972 Jul;60(1):102-19. doi: 10.1085/jgp.60.1.102.
7
Blastokinin: its size and shape, and an indication of the existence of subunits.胚动素：其大小和形状，以及亚基存在的迹象。
Fertil Steril. 1972 Feb;23(2):69-77. doi: 10.1016/s0015-0282(16)38761-1.
8
Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.人红细胞膜主要多肽的电泳分析。
Biochemistry. 1971 Jun 22;10(13):2606-17. doi: 10.1021/bi00789a030.
9
Specific protein metabolism in identifiable neurons of Aplysia californica.
J Neurobiol. 1974;5(3):243-61. doi: 10.1002/neu.480050306.