加州海兔产卵激素的生化分离与生理鉴定
Biochemical isolation and physiological identification of the egg-laying hormone in Aplysia californica.
作者信息
Arch S, Earley P, Smock T
出版信息
J Gen Physiol. 1976 Aug;68(2):197-210. doi: 10.1085/jgp.68.2.197.
Abstract
It has been determined that the bag cells of Aplysia californica produce two polypeptide species that comigrate on electrophoretic gels containing sodium dodecyl sulfate. By this separation procedure both species can be assigned a molecular weight of approximately 6,000. One of these molecules has an Rf of 0.65 on alkaline discontinuous electrophoresis gels, an isoelectric point at pH 4.8, a gel filtration molecular weight of approximately 12,000, and has no known biological function. The other does not enter alkaline disk gels, has an isoelectric point at approximately pH 9.3, shows a gel filtration molecular weight consistent with that determined by SDS gel electrophoresis, and is the egg-laying hormone.
摘要
现已确定，加州海兔的袋状细胞产生两种多肽，它们在含有十二烷基硫酸钠的电泳凝胶上迁移率相同。通过这种分离方法，这两种多肽的分子量都约为6000。其中一种分子在碱性不连续电泳凝胶上的Rf为0.65，等电点为pH 4.8，凝胶过滤分子量约为12000，且没有已知的生物学功能。另一种则不进入碱性圆盘凝胶，等电点约为pH 9.3，其凝胶过滤分子量与SDS凝胶电泳测定的结果一致，它就是产卵激素。
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Biosynthesis of the egg-laying hormone (ELH) in the bag cell neurons of Aplysia californica.加州海兔袋状细胞神经元中产卵激素（ELH）的生物合成。
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