Sharma R K, Guraya S S
Department of Zoology, Punjab Agricultural University, Ludhiana, India.
Indian J Exp Biol. 1997 Nov;35(11):1166-9.
Degenerative changes in membrana granulosa of ovaries in R. rattus have been studied using scanning electron microscopy. Ovaries from rats treated with atropine (300 mg/kg body weight) and testosterone propionate (10 IU) were used to study sequential course of atresia in granulosa cells. Granulosa cells undergoing atresia showed degenerative changes in following order i) loosening of intercellular matrix, ii) changed morphology and texture of secretory granules, iii) destabilization of granulosa cell membranes, iv) erosion of cell membrane, v) formation of specific degenerative belts, vi) pycnosis, vii) ghost cell formation and their subsequent mixing in hazzy follicular fluid of cyst. Phenomenon of atresia, its duration, course and underlying causes have been discussed.
利用扫描电子显微镜研究了黑家鼠卵巢颗粒膜的退行性变化。用阿托品（300毫克/千克体重）和丙酸睾酮（10国际单位）处理过的大鼠卵巢用于研究颗粒细胞闭锁的连续过程。发生闭锁的颗粒细胞呈现出以下顺序的退行性变化：i）细胞间基质疏松；ii）分泌颗粒的形态和质地改变；iii）颗粒细胞膜不稳定；iv）细胞膜侵蚀；v）形成特定的退行性带；vi）核固缩；vii）幽灵细胞形成以及它们随后混入囊肿的模糊卵泡液中。文中讨论了闭锁现象、其持续时间、过程及潜在原因。
