Knight R H, Van Etten J L
J Gen Microbiol. 1976 Aug;96(2):257-67. doi: 10.1099/00221287-95-2-257.
During the initial phases of germination of Botryodiplodia theobromae pycnidiospores, protein synthesis was initiated in the absence of detectable ribonucleic acid (RNA) synthesis. The lack of RNA synthesis during the early stages of germination was not an artefact due to reduced precursor permeability or to changes in the nucleotide pool. Further studies showed that the synthesis of messenger RNA and transfer RNA began about 30 min after the initiation of germination and that of 5S RNA after 45 min, whereas ribosomal RNA synthesis was not detected until after 1 h of germination. Approximately 45 to 55% of the messenger-like RNA synthesized during the first hour of germination was devoid of polyadenylate segments.
在可可球二孢壳孢子萌发的初始阶段，蛋白质合成在未检测到核糖核酸（RNA）合成的情况下就已启动。萌发早期RNA合成的缺乏并非是由于前体通透性降低或核苷酸库变化导致的假象。进一步研究表明，信使RNA和转运RNA的合成在萌发开始后约30分钟开始，5S RNA的合成在45分钟后开始，而核糖体RNA的合成直到萌发1小时后才被检测到。在萌发的第一个小时内合成的类信使RNA中，约45%至55%没有聚腺苷酸片段。
