粗糙脉孢菌分生孢子萌发过程中含聚腺苷酸核糖核酸的合成。
Synthesis of polyadenylic acid-containing ribonucleic acid during the germination of Neurospora crassa conidia.
作者信息
Mirkes P E, McCalley B
出版信息
J Bacteriol. 1976 Jan;125(1):174-80. doi: 10.1128/jb.125.1.174-180.1976.
Abstract
Ribonucleic acid (RNA) synthesized during the first 1 h of conidial germination (15 to 20, 25 to 30, and 55 to 60 min) has been characterized by sucrose-sodium dodecyl sulfate gradient centrifugation, binding to polyuridylic acid filters, and oligo(dT)-cellulose chromatography. At all labeling periods examined, polyadenylic acid-containing RNA is synthesized, processed, and incorporated into polysomes. Approximately 40% of the labeled RNA sedimenting between 5 and 17S binds to polyuridylic acid filters. RNA which binds to oligo(dT)-cellulose displays a heterogeneous distribution in sucrose-sodium dodecyl sulfate gradients with a major, broad peak at 10-16S. In addition, some polyadenylic acid-containing RNA sediments beyond the 25S marker. Approximately 3% of the [3H]adenosine in pulse-labeled polysomal RNA is in polyadenylic acid segments resistant to pancreatic and T1 ribonucleases.
摘要
在分生孢子萌发的最初1小时（15至20分钟、25至30分钟以及55至60分钟）期间合成的核糖核酸（RNA），已通过蔗糖 - 十二烷基硫酸钠梯度离心、与聚尿苷酸滤膜结合以及寡聚（dT）-纤维素层析进行了表征。在所有检测的标记时间段内，含聚腺苷酸的RNA被合成、加工并整合到多核糖体中。沉降在5至17S之间的标记RNA中，约40%与聚尿苷酸滤膜结合。与寡聚（dT）-纤维素结合的RNA在蔗糖 - 十二烷基硫酸钠梯度中呈现出异质分布，在10 - 16S处有一个主要的宽峰。此外，一些含聚腺苷酸的RNA沉降超过25S标记物。脉冲标记的多核糖体RNA中约3%的[³H]腺苷存在于对胰核糖核酸酶和T1核糖核酸酶有抗性的聚腺苷酸片段中。
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