Konukoğlu D, Cetinkale O, Bulan R
Department of Biochemistry, Cerrahpasa Medical Faculty, Istanbul University, Turkey.
Burns. 1997 Nov-Dec;23(7-8):541-4. doi: 10.1016/s0305-4179(97)00059-4.
This study was designed to determine the effect of N-acetyl-cysteine (NAC, a natural hydroxyl radical scavenger) treatment on levels of pulmonary malondialdehyde (MDA, the end product of lipid peroxidation) and glutathione (GSH, a natural antioxidant) in thermally injured rats. Severe skin scald injury (30 percent TBSA) caused a significant decrease in GSH levels, and a significant increase in MDA levels in lung tissue both at 1 h and 1 day postburn injury. Treatment of rats with NAC (15 mg/kg intraperitoneally, 15 min and 12 h following the burn) significantly improved GSH levels, and decreased ongoing lipid peroxidation at 1 day. This study showed that thermal injury resulted in increased pulmonary lipid peroxidation, and this remote organ injury was decreased by treatment with NAC. In addition NAC, a scavenger of hydroxyl radicals, improved GSH levels in the lungs. The higher level of GSH in the lungs of the burned rats treated with NAC could be due to either a decrease in the rate of degradation of GSH or to an increase in its synthesis. No data about these possibilities are provided.
本研究旨在确定N-乙酰半胱氨酸（NAC，一种天然羟自由基清除剂）治疗对热损伤大鼠肺组织中丙二醛（MDA，脂质过氧化终产物）水平和谷胱甘肽（GSH，一种天然抗氧化剂）水平的影响。严重皮肤烫伤损伤（30%体表面积）导致烧伤后1小时和1天时肺组织中GSH水平显著降低，MDA水平显著升高。用NAC（15mg/kg腹腔注射，烧伤后15分钟和12小时）治疗大鼠可显著提高GSH水平，并在1天时减少正在进行的脂质过氧化。本研究表明，热损伤导致肺脂质过氧化增加，而NAC治疗可减轻这种远隔器官损伤。此外，作为羟自由基清除剂的NAC可提高肺组织中的GSH水平。用NAC治疗的烧伤大鼠肺组织中较高的GSH水平可能是由于GSH降解速率降低或其合成增加。关于这些可能性未提供相关数据。
