Peluso M, Airoldi L, Armelle M, Martone T, Coda R, Malaveille C, Giacomelli G, Terrone C, Casetta G, Vineis P
Servizio di Oncologia Sperimentale, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
Cancer Epidemiol Biomarkers Prev. 1998 Apr;7(4):341-6.
We conducted a case-control study on 114 bladder cancer patients and 46 hospital controls. DNA adducts were measured in WBCs by 32P postlabeling and showed no association with smoking habits and the glutathione-S-transferase M1 genotype. A strong association between adduct levels and the N-acetyltransferase (NAT2) genotype was found (P = 0.0002). The NAT2 genotype was associated in a nonstatistically significant way to the case-control status (odds ratio, 1.6; 95% confidence interval, 0.8-3.2). In a logistic regression model, the log of DNA adduct levels was associated in a highly significant way to the risk of bladder cancer (regression coefficient, 0.75; P = 0.0006), independently of smoking habits. Using the median of DNA adducts (RAL, 0.3) as a cutoff point, the odds ratio for the risk of bladder cancer was 4.1 (age-adjusted; 95% confidence interval, 1.9-9.0). Our study suggests that sources other than tobacco smoke contribute to the formation of aromatic DNA adducts in WBCs. The role of WBC-DNA adducts in predicting bladder cancer is still to be clarified.
我们对114例膀胱癌患者和46例医院对照者进行了一项病例对照研究。通过32P后标记法测量白细胞中的DNA加合物，结果显示其与吸烟习惯及谷胱甘肽-S-转移酶M1基因型无关。发现加合物水平与N-乙酰基转移酶（NAT2）基因型之间存在强关联（P = 0.0002）。NAT2基因型与病例对照状态的关联无统计学意义（优势比，1.6；95%置信区间，0.8 - 3.2）。在逻辑回归模型中，DNA加合物水平的对数与膀胱癌风险高度显著相关（回归系数，0.75；P = 0.0006），且与吸烟习惯无关。以DNA加合物的中位数（相对加合物水平，0.3）作为截断点，膀胱癌风险的优势比为4.1（年龄调整后；95%置信区间，1.9 - 9.0）。我们的研究表明，除烟草烟雾外的其他来源也会导致白细胞中芳香族DNA加合物的形成。白细胞DNA加合物在预测膀胱癌中的作用仍有待阐明。
