Naves Díaz M L, Elorriaga R, Canteros A, Cannata Andía J B
Instituto Reina Sofía de Investigación Nefrológica, Hospital Central de Asturias, Departamento de Medicina, Universidad de Oviedo, Spain.
Nephrol Dial Transplant. 1998;13 Suppl 3:23-8. doi: 10.1093/ndt/13.suppl_3.23.
In the present work, we studied: (i) the effect of different doses of desferrioxamine and deferiprone on the proliferation of osteoblasts and the possible role of iron in this; and (ii) the effect of both chelators on the metabolic activity of these cells, using the quantification of alkaline phosphatase as an enzymatic marker of cellular activity or differentiation. Cellular proliferation was investigated at different concentrations of deferiprone and desferrioxamine, and the effect of the addition of iron citrate on this proliferation was measured. The production of alkaline phosphatase after incubation with deferiprone (150 and 300 microM) and desferrioxamine (20 and 100 microM) was also studied. Cellular proliferation was completely inhibited with 100 microM of desferrioxamine and 300 microM of deferiprone. In both cases, this effect was corrected by means of co-incubation with iron citrate. In the second phase, using the same dose that inhibited the proliferation, it was observed that after 24 h, both chelators slightly decreased alkaline phosphatase activity, while at 48 and 96 h they increased alkaline phosphatase activity. These results demonstrate that both desferrioxamine and deferiprone inhibit the proliferation of the osteoblast-like cell line MG-63; the effect seems to be related to the chelation of some fraction of available iron. In spite of the effect on bone cell proliferation, the chelators do not impair cellular activity.
在本研究中，我们研究了：（i）不同剂量的去铁胺和地拉罗司对成骨细胞增殖的影响以及铁在其中可能发挥的作用；（ii）使用碱性磷酸酶定量作为细胞活性或分化的酶标志物，研究这两种螯合剂对这些细胞代谢活性的影响。研究了不同浓度的地拉罗司和去铁胺对细胞增殖的影响，并测定了添加柠檬酸铁对这种增殖的作用。还研究了用150和300微摩尔/升的地拉罗司以及20和100微摩尔/升的去铁胺孵育后碱性磷酸酶的产生情况。100微摩尔/升的去铁胺和300微摩尔/升的地拉罗司完全抑制了细胞增殖。在这两种情况下，通过与柠檬酸铁共同孵育可纠正这种作用。在第二阶段，使用抑制增殖的相同剂量，观察到24小时后，两种螯合剂均轻微降低了碱性磷酸酶活性，而在48小时和96小时时它们增加了碱性磷酸酶活性。这些结果表明，去铁胺和地拉罗司均抑制成骨样细胞系MG-63的增殖；这种作用似乎与螯合一部分可利用铁有关。尽管对骨细胞增殖有影响，但螯合剂并不损害细胞活性。
