Cheng C P, Tzafrir I, Lockhart B E, Olszewski N E
Department of Plant Biology, Plant Molecular Genetics Institute, University of Minnesota, St Paul 55108, USA.
J Gen Virol. 1998 Apr;79 ( Pt 4):925-9. doi: 10.1099/0022-1317-79-4-925.
Tubular structures containing bacilliform virions were observed in cell-free extracts of Commelina diffusa infected with Commelina yellow mottle badnavirus (CoYMV). The exterior of the tubule reacted with antibodies to CoYMV movement protein, but not with antibodies to virus coat protein. Similar tubular structures containing bacilliform particles were also observed in ultrathin sections of CoYMV-infected C. diffusa. These tubular structures traversed the cell wall at points where this was thickened or protruded. No similar structures were observed in healthy C. diffusa. These observations support the hypothesis that the virion-containing tubular structures observed in cell-free extracts are the same as those observed in situ, that these structures are composed, at least in part, of virus movement protein, and that they play a role in the cell-to-cell trafficking of virions of CoYMV.
在感染了鸭跖草黄斑驳杆状DNA病毒(CoYMV)的鸭跖草无细胞提取物中观察到含有杆状病毒粒子的管状结构。小管的外部与针对CoYMV运动蛋白的抗体发生反应,但不与针对病毒外壳蛋白的抗体发生反应。在CoYMV感染的鸭跖草的超薄切片中也观察到了含有杆状颗粒的类似管状结构。这些管状结构在细胞壁增厚或突出的部位穿过细胞壁。在健康的鸭跖草中未观察到类似结构。这些观察结果支持以下假设:在无细胞提取物中观察到的含病毒粒子的管状结构与原位观察到的结构相同,这些结构至少部分由病毒运动蛋白组成,并且它们在CoYMV病毒粒子的细胞间运输中起作用。