6号染色体6Q27区域上D6S149 - D6S193区间的物理图谱及其与卵巢表面上皮良性肿瘤的关系。
Physical map of the D6S149-D6S193 region on chromosome 6Q27 and its involvement in benign surface epithelial ovarian tumours.
作者信息
Tibiletti M G, Trubia M, Ponti E, Sessa L, Acquati F, Furlan D, Bernasconi B, Fichera M, Mihalich A, Ziegler A, Volz A, Facco C, Riva C, Cremonesi L, Ferrari M, Taramelli R
机构信息
Dipartimento di Patologia, Universita' degli Studi di Pavia (sede Varese), Ospedale di Circolo Varese, Italy.
出版信息
Oncogene. 1998 Mar 26;16(12):1639-42. doi: 10.1038/sj.onc.1201654.
A detailed long range restriction map of the region defined by markers D6S149 and D6S193 on chromosome 6q27 has been constructed. This was achieved by YAC cloning and contig assembling of the same region. Seven YAC clones were found to span the almost 1000 Kb region flanked by the two markers which on the genetic map resulted to be 1.9 cM apart. With some of the characterized YAC clones we undertook a molecular cytogenetic analysis of 20 benign ovarian tumors. The rationale for this was the recent mapping to a region of chromosome 6q27, flanked by markers D6281 and D6S133, of a locus for the SV40-mediated immortalization of human cells (SEN6 gene). Noteworthy we found that the the D6S149-D6S193 region (comprised in the larger D6S281-D6S133 physical interval) was altered in all samples analysed adding support to the occurrence of a immortalization step in this type of tumors.
已构建出由6号染色体6q27上的标记D6S149和D6S193所定义区域的详细长程限制酶切图谱。这是通过该区域的酵母人工染色体（YAC）克隆和重叠群组装实现的。发现7个YAC克隆跨越了由这两个标记侧翼的近1000千碱基区域，在遗传图谱上这两个标记相距1.9厘摩。利用一些已鉴定的YAC克隆，我们对20例良性卵巢肿瘤进行了分子细胞遗传学分析。这样做的理由是最近将人类细胞的SV40介导永生化位点（SEN6基因）定位到了6号染色体6q27上一个由标记D6281和D6S133侧翼的区域。值得注意的是，我们发现在所有分析的样本中，D6S149 - D6S193区域（包含在更大的D6S281 - D6S133物理区间内）都发生了改变，这为这类肿瘤中存在永生化步骤提供了支持。
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