Buttitta F, Marchetti A, Radi O, Bertacca G, Pellegrini S, Gadducci A, Genazzani A R, Bevilacqua G
Department of Oncology, University of Pisa, Italy.
Br J Cancer. 1998 Apr;77(7):1048-51. doi: 10.1038/bjc.1998.175.
The FHIT gene, recently cloned and mapped on chromosome 3p14.2, has frequently been found to be abnormal in several established cancer cell lines and primary tumours. As alterations of chromosome 3p are common events in ovarian cancers with breakpoint sites at 3p14.2, we decided to investigate the role of FHIT in human ovarian tumorigenesis. Fifty-four primary ovarian carcinomas were studied by reverse transcription of FHIT mRNA followed by polymerase chain reaction (PCR) amplification and sequencing of products. The same tumours and matched normal tissues were also investigated for loss of heterozygosity using three microsatellite markers located inside the gene. We found an abnormal transcript of the FHIT gene in two cases (4%) and allelic losses in eight cases (15%). Twelve (22%) of the 54 tumours investigated belonged to young patients with a family history of breast/ovarian cancer. In none of these cases was the FHITgene found to be altered. Our results indicate that FHITplays a role in a small proportion of ovarian carcinomas.
FHIT基因最近被克隆并定位于染色体3p14.2上，人们经常发现它在几种已建立的癌细胞系和原发性肿瘤中存在异常。由于3p染色体改变是卵巢癌中的常见事件，其断点位于3p14.2，我们决定研究FHIT在人类卵巢肿瘤发生中的作用。通过对FHIT mRNA进行逆转录，随后进行聚合酶链反应（PCR）扩增及产物测序，对54例原发性卵巢癌进行了研究。还使用位于该基因内部的三个微卫星标记，对相同肿瘤及配对的正常组织进行杂合性缺失研究。我们在两例（4%）中发现了FHIT基因的异常转录本，在八例（15%）中发现了等位基因缺失。在接受调查的54例肿瘤中，有12例（22%）属于有乳腺癌/卵巢癌家族史的年轻患者。在这些病例中，均未发现FHIT基因发生改变。我们的结果表明，FHIT在一小部分卵巢癌中发挥作用。
