Jacobs W, Bogers J J, Timmermans J P, Deelder A M, Van Marck E A
Department of Pathology, University of Antwerp, Wilrijk, Belgium.
Parasitol Res. 1998;84(4):276-80. doi: 10.1007/s004360050395.
Adhesion molecules constitute essential elements in inflammation, mediating various cellular interactions. We investigated the expression of adhesion molecules mediating cell-cell [intercellular adhesion molecule-1 (ICAM-1) and lymphocyte function-associated antigen-1 (LFA-1)] and cell-matrix interactions [very late antigen-4 (VLA-4), VLA-6, and syndecan-1] in intestinal granulomas of mice infected with the parasite Schistosoma mansoni. Up-regulation of ICAM-1, LFA-1, and VLA-4 was seen in ileal and colonic granulomas, at both the acute (8 weeks postinfection) and the chronic stage (13-16 weeks postinfection). Up-regulation of VLA-6 was absent in all intestinal granulomas. Syndecan-1 immunoreactive (antigen-driven) B-lymphocytes were seen in the proximity of egg-antigen-laden macrophages in the inner part of ileal and colonic granulomas, although B-cells are considered to be absent in ileal granulomas. Estimation of intestinal granuloma volumes demonstrated the lack of down-modulation observed in ileal granulomas. From our results we infer that adhesion molecules constitute important elements in schistosomal intestinal granuloma formation. Organ-related differences between hepatic and intestinal granulomas exist (e.g., granuloma volume), but these differences are not morphologically reflected in a differential expression of the adhesion molecules ICAM-1, LFA-1, and VLA-4. Syndecan-1 immunoreactive B-lymphocytes also appear to be involved in ileal granuloma formation.
黏附分子是炎症中的关键要素，介导多种细胞间相互作用。我们研究了在感染曼氏血吸虫的小鼠肠道肉芽肿中介导细胞-细胞相互作用（细胞间黏附分子-1（ICAM-1）和淋巴细胞功能相关抗原-1（LFA-1））以及细胞-基质相互作用（极迟抗原-4（VLA-4）、VLA-6和syndecan-1）的黏附分子的表达情况。在急性感染期（感染后8周）和慢性感染期（感染后13 - 16周），回肠和结肠肉芽肿中均可见ICAM-1、LFA-1和VLA-4上调。所有肠道肉芽肿中均未出现VLA-6上调。在回肠和结肠肉芽肿内部，富含虫卵抗原的巨噬细胞附近可见syndecan-1免疫反应性（抗原驱动）B淋巴细胞，尽管通常认为回肠肉芽肿中不存在B细胞。肠道肉芽肿体积的评估显示回肠肉芽肿中未观察到下调现象。从我们的结果推断，黏附分子是血吸虫性肠道肉芽肿形成的重要因素。肝肉芽肿和肠道肉芽肿之间存在器官相关差异（如肉芽肿体积），但这些差异在黏附分子ICAM-1、LFA-1和VLA-4的差异表达中未在形态学上得到体现。syndecan-1免疫反应性B淋巴细胞似乎也参与回肠肉芽肿的形成。
