大鼠体内身体成分研究：全身蛋白质评估

In vivo body composition studies in rats: assessment of total body protein.

作者信息

Yasumura S, Stamatelatos I E, Boozer C N, Moore R, Ma R

机构信息

Medical Department, Brookhaven National Laboratory, Upton, NY 11973, USA.

出版信息

Appl Radiat Isot. 1998 May-Jun;49(5-6):731-2. doi: 10.1016/s0969-8043(97)00209-1.

DOI:10.1016/s0969-8043(97)00209-1

PMID:9569593

Abstract

The precision and accuracy of a prompt-gamma neutron activation facility developed to assess total body protein in rats is estimated. The coefficient of variation of nitrogen measurement, as estimated by repeated measurements on 15 rats, was 5.5% for an equivalent dose of 60 mSv (Q = 20). Good agreement was observed in comparing the results of in vivo neutron activation analysis and chemical carcass analysis performed by the Kjeldahl method. The application of the technique in comparing the effect of a low-fat and a high-fat diet on body protein in rats is demonstrated.

摘要

对为评估大鼠全身蛋白质而开发的瞬发γ中子活化设备的精度和准确性进行了估算。通过对15只大鼠进行重复测量估算得出，在等效剂量为60毫希沃特（品质因数Q = 20）时，氮测量的变异系数为5.5%。在比较体内中子活化分析结果与采用凯氏定氮法进行的化学胴体分析结果时，观察到两者具有良好的一致性。展示了该技术在比较低脂饮食和高脂饮食对大鼠体内蛋白质影响方面的应用。